Separation of components of human globulins by capillary zone electrophoresis using a linear polyacrylamide-coated capillary

• Published in: Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 1997-09, Vol.697 (1), p.217-222
• Main Author: Kubo, Kanenobu
• Format: Article
• Language: English
• Subjects: Acrylic Resins; Electrophoresis, Capillary - instrumentation; Globulins; Humans; Hydrogen-Ion Concentration; Serum Globulins - analysis
• ISSN: 0378-4347; 1387-2273
• DOI: 10.1016/S0378-4347(97)00131-X

Eleven commercially available protein preparations from human serum were subjected to capillary zone electrophoresis (CZE) using a linear polyacrylamide-coated capillary at pH 7.4. Transferrin, complement C3 and C-reactive protein were each separated into one major peak and several minor peaks. α 1-Antitrypsin was separated into two major peaks and three minor peaks. α 2-HS-glycoprotein showed four major peaks with a leading shoulder. Haptoglobin, α 2-microglobulin, α 1-acid-glycoprotein and prealbumin were detected as relatively wide peaks. Ceruloplasmin showed one major peak with notches, and two minor and several notched peaks. Only low density lipoprotein showed no peaks. A mixture of five of the protein preparations was separated into individual components, as well as individual isoforms. When the same mixture was analyzed by CZE using an uncoated capillary, a much poorer resolution was obtained. Application of this CZE system to albumin-depleted serum demonstrated that it is very useful for analyzing globulin components in serum.