Analysis of low-molecular-mass proteins in cerebrospinal fluid by sodium dodecyl sulfate capillary gel electrophoresis

• Published in: Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 1997-09, Vol.697 (1), p.141-147
• Main Authors: Hiraoka, Atsushi, Arato, Teruyo, Tominaga, Itaru, Eguchi, Naomi, Oda, Hiroshi, Urade, Yoshihiro
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Central nervous system disease; Cerebrospinal fluid; Cerebrospinal Fluid Proteins - analysis; Cerebrospinal Fluid Proteins - chemistry; Electrophoresis, Capillary; Female; Gels; Humans; Male; Middle Aged; Molecular Weight; Myelin basic protein; Proteins; Reproducibility of Results; Sodium Dodecyl Sulfate; α1-Acid glycoprotein; β-Trace protein; β2-Microglobulin
• ISSN: 0378-4347; 1387-2273
• DOI: 10.1016/S0378-4347(97)00326-5

Proteins of low molecular mass ( M r) in human cerebrospinal fluid (CSF) were analyzed by capillary electrophoresis in a sodium dodecyl sulfate-containing polymer solution. Under the conditions employed, peaks of β 2-microglobulin (βMG) ( M r: 11 700), γ-trace protein (12 300), myelin basic protein (18 000), β-trace protein (βTP) (23 000 to 30 000) and α 1-acid glycoprotein (42 000) were detected on the electropherograms. The concentrations of βMG and βTP were determined based on the peak area relative to that of an internal standard, Orange G, which was added at a constant amount as the front marker. It was demonstrated that their levels in CSF change under various pathological conditions in the central nervous system.