Heat Shock Factor 1 Represses Ras-induced Transcriptional Activation of the c-fos Gene

Heat shock factor 1, the critical molecular regulator of the stress response is conserved throughout eukaryotic organisms and activates the transcription of heat shock genes. We now show that heat shock factor 1 inhibits the expression of c-fos, an immediate early gene that controls responses to ext...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 1997-10, Vol.272 (43), p.26803-26806
Main Authors: Chen, Changmin, Xie, Yue, Stevenson, Mary Ann, Auron, Philip E., Calderwood, Stuart K.
Format: Article
Language:English
Subjects:
Animals
beta-Galactosidase - biosynthesis
CHO Cells
Cricetinae
DNA-Binding Proteins - biosynthesis
DNA-Binding Proteins - metabolism
Genes, fos
Genes, Reporter
Heat Shock Transcription Factors
Heat-Shock Proteins - biosynthesis
Heat-Shock Proteins - metabolism
Humans
Luciferases - biosynthesis
Promoter Regions, Genetic
Proto-Oncogene Proteins c-fos - biosynthesis
Proto-Oncogene Proteins p21(ras) - biosynthesis
Proto-Oncogene Proteins p21(ras) - metabolism
Recombinant Fusion Proteins - biosynthesis
Transcription Factors - biosynthesis
Transcription Factors - metabolism
Transcription, Genetic
Transfection
Urokinase-Type Plasminogen Activator - biosynthesis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Heat shock factor 1, the critical molecular regulator of the stress response is conserved throughout eukaryotic organisms and activates the transcription of heat shock genes. We now show that heat shock factor 1 inhibits the expression of c-fos, an immediate early gene that controls responses to extracellular stimuli for growth and differentiation. Heat shock factor 1 inhibits the transcription of the c-fos gene and antagonizes the activating effects of the signal transducing protein Ras on the c-fos promoter and on the promoter of another Ras responsive gene uPA. This property was specific for heat shock factor 1; c-fos repression was not seen with the structurally related protein heat shock factor 2. Repression involved different molecular mechanisms compared with those involved in transcriptional activation by heat shock factor 1 and specifically did not require binding to the c-fos promoter. Thus, in addition to its known role as a transcriptional activator of the cellular heat shock response, heat shock factor 1 also antagonizes the expression of Fos, a key component of the ubiquitous AP-1 transcription factor complex and as such could influence multiple aspects of cell regulation.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.272.43.26803