Recombinant human antibodies specific for hepatitis C virus proteins

Human antibodies to hepatitis C virus core, NS4A and NS3 were cloned in a prokaryotic vector and expressed as soluble Fab fragments and as phage-displayed Fabs. The recombinant Fabs were shown to be a suitable tool for immunohistochemistry, since they recognize the cognate antigen expressed in mamma...

Full description

Saved in:
Bibliographic Details
Published in:Archives of virology 1997-01, Vol.142 (3), p.601-610
Main Authors: Esposito, G, Morea, V, Scarselli, E, Cerino, A, Mondelli, M. U, La Monica, N, Traboni, C
Format: Article
Language:English
Subjects:
amino acid sequences
antibodies
Antibody Specificity - immunology
antigens
Base Sequence
Cell Line
Cloning, Molecular
complementary DNA
DNA, Complementary
Hepatitis
Hepatitis C Antibodies - chemistry
Hepatitis C Antibodies - genetics
Hepatitis C Antibodies - immunology
Hepatitis C Antigens - immunology
Hepatitis C virus
Humans
Immunoglobulin Fab Fragments - genetics
Immunoglobulin Fab Fragments - immunology
Immunoglobulin Variable Region - chemistry
immunohistochemistry
Models, Molecular
Molecular Sequence Data
nucleotide sequences
Protein Conformation
Proteins
Recombinant Fusion Proteins - immunology
Viral Core Proteins - immunology
Viral Nonstructural Proteins - immunology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Human antibodies to hepatitis C virus core, NS4A and NS3 were cloned in a prokaryotic vector and expressed as soluble Fab fragments and as phage-displayed Fabs. The recombinant Fabs were shown to be a suitable tool for immunohistochemistry, since they recognize the cognate antigen expressed in mammalian cells. The nucleotide sequence of the cDNA for the variable domains of these antibodies was determined and the V-gene usage was derived. On the basis of the deduced amino acid sequence, a structural model of the V domains of the Fabs was constructed.
ISSN:0304-8608
1432-8798
DOI:10.1007/s007050050106