Map-based cloning of the tomato genomic region that spans the Sw-5 tospovirus resistance gene in tomato

Two yeast artificial chromosomes (YACs) containing genomic DNA from tomato have been isolated using CT220, an RFLP marker which is tightly linked to the tomato spotted wilt virus resistance gene, Sw-5. High-resolution mapping of the YAC ends and internal YAC probes demonstrated that one of the YAC c...

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Bibliographic Details
Published in:Molecular & general genetics 1997-09, Vol.256 (2), p.121-126
Main Authors: Brommonschenkel, S.H, Tanksley, S.D
Format: Article
Language:English
Subjects:
Chromosome Mapping
Chromosomes, Artificial, Yeast - genetics
cloning
Cloning, Molecular
Cosmids
disease resistance
DNA libraries
DNA, Plant - genetics
Gene Library
genes
Genes, Plant
genetic markers
genetic recombination
Immunity, Innate - genetics
loci
Lycopersicon esculentum - genetics
Lycopersicon esculentum - virology
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Recombination, Genetic
restriction fragment length polymorphism
Solanum lycopersicum var. lycopersicum
sur5 gene
sur5 locus
Tomato spotted wilt virus
Tospovirus - genetics
Tospovirus - pathogenicity
yeast artificial chromosomes
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Summary:Two yeast artificial chromosomes (YACs) containing genomic DNA from tomato have been isolated using CT220, an RFLP marker which is tightly linked to the tomato spotted wilt virus resistance gene, Sw-5. High-resolution mapping of the YAC ends and internal YAC probes demonstrated that one of the YAC clones, TY257 (400 kb), spans Sw-5. By chromosome walking in a cosmid library, the position of Sw-5 has been delimited within the YAC to a maximal chromosomal segment of 100 kb, spanned by nine overlapping cosmid clones.
ISSN:0026-8925
1432-1874
DOI:10.1007/s004380050553