Purification of recombinant human precursor acid α‐glucosidase

Large quantities of recombinant acid α‐glucosidase are needed for in viva experimentation of enzyme replacement therapy in Pompe disease. We describe a new purification method for the purification of this recombinant enzyme from tissue culture medium consisting of concanavalin A affinity chromatogra...

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Bibliographic Details
Published in:Biochemistry and molecular biology international 1997-10, Vol.43 (3), p.613-623
Main Authors: Van Hove, Johan L. K., Yang, Helen W., Oliver, Letealia M., Pennybacker, Mark F., Chen, Yuan‐Tsong
Format: Article
Language:English
Subjects:
Acid α‐glucosidase
alpha-Glucosidases - isolation & purification
alpha-Glucosidases - metabolism
alpha-Glucosidases - pharmacology
Animals
CHO Cells - enzymology
Chromatography - methods
Cricetinae
Fibroblasts - drug effects
Glycogen Storage Disease Type II - enzymology
Humans
Pompe disease
protein purification
recombinant enzyme
subfractions
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Summary:Large quantities of recombinant acid α‐glucosidase are needed for in viva experimentation of enzyme replacement therapy in Pompe disease. We describe a new purification method for the purification of this recombinant enzyme from tissue culture medium consisting of concanavalin A affinity chromatography, hydrophobic interaction chromatography, affinity chromatography on Superdex, and anion exchange chromatography. The new method is amenable to scale up, and has increased speed, and improved reproducibility with similar high yield and purification efficiency when compared to previous methods.
ISSN:1521-6543
1039-9712
1521-6551
DOI:10.1080/15216549700204421