Radiolabeling of monoclonal antibody B43.13 with rhenium-188 for immunoradiotherapy

In this study we report a novel method for direct radiolabeling of monoclonal antibody B43.13 (MAb-B43.13) with 188Re and have evaluated the product's radiochemical, biochemical, immunochemical and selected biological properties. 188ReMAbB43.13 was readily prepared by the addition of generato...

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Bibliographic Details
Published in:Applied radiation and isotopes 1997-07, Vol.48 (7), p.899-906
Main Authors: Sykes, Thomas R., Somayaji, Vijayalakshmi V., Bier, Stefan, Woo, Thomas K., Kwok, Cheuk S., Snieckus, Victor, Noujaim, Antoine A.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - pharmacokinetics
Cell Line
Drug Stability
Female
Humans
Mice
Mice, Inbred BALB C
Mice, SCID
Ovarian Neoplasms - metabolism
Radioimmunotherapy
Radioisotopes - pharmacokinetics
Rhenium - pharmacokinetics
Tissue Distribution
Transplantation, Heterologous
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Summary:In this study we report a novel method for direct radiolabeling of monoclonal antibody B43.13 (MAb-B43.13) with 188Re and have evaluated the product's radiochemical, biochemical, immunochemical and selected biological properties. 188ReMAbB43.13 was readily prepared by the addition of generator produced perrhenate to a preformulated antibody vial after an optimal amount of supplemental stannous ion, in the form of stannous tartrate, was added. The final radiolabeled product retained its biochemical purity (as determined by size-exclusion HPLC and R/NR-SDS-PAGE), its immunoreactivity (as determined by immunoassay) and presented with a typical stability (in the presence of serum and cysteine) and biodistribution (in tumored mice) profile. The evaluation of the product for immunoradiotherapy of ovarian cancer in a clinical setting requires further studies.
ISSN:0969-8043
1872-9800
DOI:10.1016/S0969-8043(97)00025-0