The effect of chorionic villus sampling on the number of fetal cells isolated from maternal blood and on maternal serum alpha-fetoprotein levels

Fetal cells are present in the circulation of pregnant women and can be isolated using density gradient centrifugation and magnetic cell sorting. In the present study, maternal cell preparations were depleted for CD45‐ and CD14‐positive cells and enriched for CD71‐positive cells. The number of fetal...

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Bibliographic Details
Published in:Prenatal diagnosis 1997-10, Vol.17 (10), p.953-959
Main Authors: Jansen, Mieke W. J. C., Brandenburg, Helen, Wildschut, Hajo I. J., Martens, Anton C. M., Hagenaars, Adriana M., Wladimiroff, Juriy W., In 't Veld, Peter A.
Format: Article
Language:English
Subjects:
Adult
alpha-fetoprotein
alpha-Fetoproteins - metabolism
Biological and medical sciences
Chorionic Villi Sampling - adverse effects
Chorionic Villi Sampling - methods
chorionic villus sampling
Erythroid Precursor Cells - metabolism
Female
fetal cells
feto-maternal transfusion
Fetomaternal Transfusion - blood
Gestational Age
Gynecology. Andrology. Obstetrics
Humans
In Situ Hybridization, Fluorescence
Karyotyping
Male
Management. Prenatal diagnosis
maternal blood
Medical sciences
Pregnancy
Pregnancy. Fetus. Placenta
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Summary:Fetal cells are present in the circulation of pregnant women and can be isolated using density gradient centrifugation and magnetic cell sorting. In the present study, maternal cell preparations were depleted for CD45‐ and CD14‐positive cells and enriched for CD71‐positive cells. The number of fetal nucleated cells was determined using fluorescence in situ hybridization for X and Y chromosomes. Analysis of maternal blood samples taken before and after transabdominal chorionic villus sampling (TA‐CVS) showed an increase in the number of fetal cells in 10 out of 19 male pregnancies after the invasive procedure. This cellular transfusion was found to correlate with elevated maternal serum alpha‐fetoprotein levels. TA‐CVS‐induced cellular transfusion may form a good in vivo system to optimize fetal cell isolation procedures and to study fetal cell dynamics and characteristics. © 1997 John Wiley & Sons, Ltd.
ISSN:0197-3851
1097-0223
DOI:10.1002/(SICI)1097-0223(199710)17:10<953::AID-PD182>3.0.CO;2-E