Human Prk Is a Conserved Protein Serine/Threonine Kinase Involved in Regulating M Phase Functions

Human prk encodes a novel protein serine/threonine kinase capable of strongly phosphorylating casein but not histone H1 in vitro. prk expression is tightly regulated at various levels during different stages of the cell cycle in lung fibroblasts. The Prk kinase activity is relatively low during mito...

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Bibliographic Details
Published in:The Journal of biological chemistry 1997-11, Vol.272 (45), p.28646-28651
Main Authors: Ouyang, Bin, Pan, Huiqi, Lu, Luo, Li, Jian, Stambrook, Peter, Li, Bo, Dai, Wei
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Caseins - metabolism
cdc25 Phosphatases
Cell Cycle Proteins - metabolism
Cells, Cultured
Cloning, Molecular
DNA, Complementary - chemistry
Down-Regulation
Drosophila
Fibroblasts - enzymology
Humans
Lung Neoplasms - enzymology
Mice
Mitosis
Molecular Sequence Data
Phosphorylation
Protein Kinase C
Protein-Serine-Threonine Kinases - genetics
Protein-Serine-Threonine Kinases - metabolism
RNA, Messenger - metabolism
Xenopus laevis
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Summary:Human prk encodes a novel protein serine/threonine kinase capable of strongly phosphorylating casein but not histone H1 in vitro. prk expression is tightly regulated at various levels during different stages of the cell cycle in lung fibroblasts. The Prk kinase activity is relatively low during mitosis, G1, and G1/S, and peaks during late S and G2 stages of the cell cycle. Recombinant human Prk expressed through the baculoviral vector system is capable of phosphorylating Cdc25C, a positive regulator for the G2/M transition. Human prk shares significant sequence homology with Saccharomyces cerevisiae CDC5 and Drosophila melanogaster polo, both of which are essential for mitosis and meiosis. Full-length prk transcripts greatly potentiate progesterone-induced meiotic maturation of Xenopus laevisoocytes. On the other hand, antisense prk transcripts significantly delay and reduce the rate of oocyte maturation. When expressed in a CDC5 mutant strain of S. cerevisiae, human Prk, but not a deletional mutant protein, fully rescues the temperature-sensitive phenotype of the budding yeast. Taken together,prk may represent a new protein kinase, playing an important role in regulating the onset and/or progression of mitosis in mammalian cells.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.272.45.28646