Crosslinking of hyaluronic acid with water-soluble carbodiimide

Hyaluronic acid (HA) was chemically crosslinked with a water‐soluble carbodiimide (WSC) to produce low‐water‐content films when brought into contact with water. The crosslinking reaction was performed in two different ways; one was by using HA films and the other by casting HA solutions. Both method...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biomedical materials research 1997-11, Vol.37 (2), p.243-251
Main Authors: Tomihata, Kenji, Ikada, Yoshito
Format: Article
Language:English
Subjects:
Biodegradation
Biofilms
Biological and medical sciences
Carbodiimides - chemistry
Cross-Linking Reagents - chemistry
Crosslinking
hyaluronic acid
Hyaluronic Acid - chemistry
Infrared spectroscopy
IR spectra
Medical sciences
Nitrogen compounds
Pectins - chemistry
Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)
Solubility
Spectrophotometry, Infrared
Technology. Biomaterials. Equipments. Material. Instrumentation
Water - chemistry
water-soluble carbodiimide
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Hyaluronic acid (HA) was chemically crosslinked with a water‐soluble carbodiimide (WSC) to produce low‐water‐content films when brought into contact with water. The crosslinking reaction was performed in two different ways; one was by using HA films and the other by casting HA solutions. Both methods produced water‐insoluble HA films. The lowest water content of the crosslinked HA films subjected to swelling with water was 60 wt% at 37°C, which was lower than any reported values. Infrared spectra of the crosslinked films suggested that intermolecular formation of ester bonds between the hydroxyl and carboxyl groups belonging to different polysaccharide molecules led to crosslinking. For comparison, pectin which possesses hydroxyl and carboxyl groups in one molecule, similar to HA, was subjected to crosslinking with WSC. The finding on pectin also supported ester formation between different polysaccharide molecules. The crosslinking of HA film with WSC in the presence of L‐lysine methyl ester prolonged the in vivo degradation of HA film, probably because of amide bond formation as the crosslink. © 1997 John Wiley & Sons, Inc. J Biomed Mater Res, 37, 243–251, 1997.
ISSN:0021-9304
1097-4636
DOI:10.1002/(SICI)1097-4636(199711)37:2<243::AID-JBM14>3.0.CO;2-F