Laser Photodissociation of Insulin Ions Generated by Matrix-Assisted Laser Desorption

A novel method for studying the ionization step of matrix-assisted laser desorption/ionization (MALDI) is demonstrated. A 193-nm pulse from an ArF excimer laser is used to photodissociate a portion of a plume of insulin ions generated by MALDI. Laser photodissociation (LPD) creates a “hole”, i.e., a...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 1997-11, Vol.69 (21), p.4390-4398
Main Authors: Preisler, Jan, Yeung, Edward S
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chemistry
Fundamental and applied biological sciences. Psychology
Insulin
Insulin - analysis
Ions
Lasers
Nanotechnology
Protein hormones. Growth factors. Cytokines
Proteins
Scientific imaging
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
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Summary:A novel method for studying the ionization step of matrix-assisted laser desorption/ionization (MALDI) is demonstrated. A 193-nm pulse from an ArF excimer laser is used to photodissociate a portion of a plume of insulin ions generated by MALDI. Laser photodissociation (LPD) creates a “hole”, i.e., a negative spike in the insulin peak in the time-of-flight (TOF) mass spectrum. The position of the hole in the mass spectrum provides useful measurements of the characteristics (position, time, and velocity) of insulin ions shortly after their creation. Although the performance of the method can be further improved, the data obtained could be used to refine our current understanding of MALDI and to improve the resolution of MALDI-TOFMS.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac970248f