Association of the origin recognition complex with heterochromatin and HP1 in higher eukaryotes

The origin recognition complex (ORC) is required to initiate eukaryotic DNA replication and also engages in transcriptional silencing in S. cerevisiae. We observed a striking preferential but not exclusive association of Drosophila ORC2 with heterochromatin on interphase and mitotic chromosomes. HP1...

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Bibliographic Details
Published in:Cell 1997-10, Vol.91 (3), p.311-323
Main Authors: Pak, D T, Pflumm, M, Chesnokov, I, Huang, D W, Kellum, R, Marr, J, Romanowski, P, Botchan, M R
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Chromosomal Proteins, Non-Histone - metabolism
Cloning, Organism
DNA-Binding Proteins - metabolism
Drosophila melanogaster
Drosophila Proteins
Heterochromatin - metabolism
Molecular Sequence Data
Origin Recognition Complex
Protein Binding
Saccharomyces cerevisiae Proteins
Xenopus
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Summary:The origin recognition complex (ORC) is required to initiate eukaryotic DNA replication and also engages in transcriptional silencing in S. cerevisiae. We observed a striking preferential but not exclusive association of Drosophila ORC2 with heterochromatin on interphase and mitotic chromosomes. HP1, a heterochromatin-localized protein required for position effect variegation (PEV), colocalized with DmORC2 at these sites. Consistent with this localization, intact DmORC and HP1 were found in physical complex. The association was shown biochemically to require the chromodomain and shadow domains of HP1. The amino terminus of DmORC1 contained a strong HP1-binding site, mirroring an interaction found independently in Xenopus by a yeast two-hybrid screen. Finally, heterozygous DmORC2 recessive lethal mutations resulted in a suppression of PEV. These results indicate that ORC may play a widespread role in packaging chromosomal domains through interactions with heterochromatin-organizing factors.
ISSN:0092-8674
DOI:10.1016/S0092-8674(00)80415-8