Induction of hepatic egg granuloma hyporesponsiveness in murine schistosomiasis mansoni by intravenous injection of small doses of soluble egg antigen

This work was designed to test whether hyporesponsiveness to schistosomal egg antigen (SEA) was associated with reduction in size of hepatic granulomas. Multiple small doses of SEA (10 m̈gx4) were injected intravenously (i.v.) into C57Bl/6 mice either at 7 or 30 days prior to cercarial exposure. Eig...

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Published in:APMIS : acta pathologica, microbiologica et immunologica Scandinavica microbiologica et immunologica Scandinavica, 1997-10, Vol.105 (7-12), p.773-783
Main Authors: HASSANEIN, HANAA, AKL, MAHA, SHAKER, ZEINAB, EL-BAZ, HANAN, SHARMY, RAGIA, RABIAE, IBRAHIM, BOTROS, SANAA
Format: Article
Language:English
Subjects:
Animals
Antibodies, Helminth - biosynthesis
Antigens, Helminth - administration & dosage
Antigens, Helminth - immunology
Biological and medical sciences
Diseases caused by trematodes
Dose-Response Relationship, Immunologic
granuloma
Granuloma - immunology
Helminthic diseases
Immunoglobulin G - immunology
immunology
Immunosuppression - methods
Infectious diseases
Injections, Intravenous
Lymphocyte Activation
Medical sciences
Mice
Mice, Inbred C57BL
Parasitic diseases
pathology
S. mansoni
Schistosomiases
Schistosomiasis mansoni - immunology
Schistosomiasis mansoni - pathology
Solubility
soluble egg antigen
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Summary:This work was designed to test whether hyporesponsiveness to schistosomal egg antigen (SEA) was associated with reduction in size of hepatic granulomas. Multiple small doses of SEA (10 m̈gx4) were injected intravenously (i.v.) into C57Bl/6 mice either at 7 or 30 days prior to cercarial exposure. Eight weeks postinfection, hepatic histopathology and granuloma diameter were studied. SEA‐induced lympho‐proliferative response, splenic cytokines (IL‐2, IL‐4 and IL‐5) and serum antischistosomal IgG were assessed. Worm burden and tissue egg load were counted. Compared to infected controls, the SEA‐treated groups showed decrease in granuloma diameter, remarkable increase in the percentage of degenerated ova within hepatic granulomas and amelioration of histopathological changes. SEA lymphoproliferative response, and levels of Il‐2 and IL‐4, were lower in SEA‐treated groups than infected controls. The levels of IL‐5 and antishistosomal IgG were comparable to the infected controls. The intensity of infection was not influenced by i.v. injection of SEA. The present data show that i.v. administration of multiple small doses of SEA induced granulomatous hyporesponsiveness with amelioration of hepatic pathology and acceleration of egg destruction.
ISSN:0903-4641
1600-0463
DOI:10.1111/j.1699-0463.1997.tb05083.x