The in vivo effect of lipopolysaccharide on Na +,K +-ATPase catalytic ( α) subunit isoforms in rat sciatic nerve

• Published in: Neuroscience letters 1997-10, Vol.234 (2), p.166-168
• Main Authors: Liu, Shing H, Sheu, Tzong J
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Binding Sites; Biological and medical sciences; Catalysis; Endotoxemia - enzymology; Endotoxin; Enzyme Inhibitors - pharmacology; Fundamental and applied biological sciences. Psychology; Isoenzymes - drug effects; Lipopolysaccharides - pharmacology; Male; Na +,K +-ATPase; Nerve; Nitric oxide; Nitric Oxide - physiology; Nitroarginine - pharmacology; Peptide Fragments - drug effects; Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ; Polymyxin B - pharmacology; Rats; Rats, Wistar; Sciatic Nerve - drug effects; Sciatic Nerve - enzymology; Sodium-Potassium-Exchanging ATPase - drug effects; Vertebrates: nervous system and sense organs
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/S0304-3940(97)00686-1

The Na +, K +-ATPase catalytic ( α) subunit in sciatic nerve of lipopolysaccharide (endotoxin, LPS)-treated rat was investigated. Using Western blot to determine subunit isoform polypeptide levels in rat sciatic nerve, we found a substantial reduction in α1 polypeptide, but not that of α2 and α3 polypeptides, after the administration of LPS. Moreover, when rats were treated with polymyxin B (a LPS neutralizer) and N G-nitro- l-arginine (an inhibitor of nitric oxide (NO) synthase), the effects of LPS were reversed. These results implicate a specific marked deficit in α1 subunit isoform of Na +,K +-ATPase in the pathogenesis of neuropathy during endotoxemia, through, at least in part, the l-arginine/NO pathway.