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The in vivo effect of lipopolysaccharide on Na +,K +-ATPase catalytic ( α) subunit isoforms in rat sciatic nerve
The Na +, K +-ATPase catalytic ( α) subunit in sciatic nerve of lipopolysaccharide (endotoxin, LPS)-treated rat was investigated. Using Western blot to determine subunit isoform polypeptide levels in rat sciatic nerve, we found a substantial reduction in α1 polypeptide, but not that of α2 and α3 pol...
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| Published in: | Neuroscience letters 1997-10, Vol.234 (2), p.166-168 |
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| Main Authors: | , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c389t-9985d23b7cee517233ae625212d7035c9f93a1ad06f67e99f995a79f89fd4a583 |
| container_end_page | 168 |
| container_issue | 2 |
| container_start_page | 166 |
| container_title | Neuroscience letters |
| container_volume | 234 |
| creator | Liu, Shing H Sheu, Tzong J |
| description | The Na
+, K
+-ATPase catalytic (
α) subunit in sciatic nerve of lipopolysaccharide (endotoxin, LPS)-treated rat was investigated. Using Western blot to determine subunit isoform polypeptide levels in rat sciatic nerve, we found a substantial reduction in
α1 polypeptide, but not that of
α2 and
α3 polypeptides, after the administration of LPS. Moreover, when rats were treated with polymyxin B (a LPS neutralizer) and
N
G-nitro-
l-arginine (an inhibitor of nitric oxide (NO) synthase), the effects of LPS were reversed. These results implicate a specific marked deficit in
α1 subunit isoform of Na
+,K
+-ATPase in the pathogenesis of neuropathy during endotoxemia, through, at least in part, the
l-arginine/NO pathway. |
| doi_str_mv | 10.1016/S0304-3940(97)00686-1 |
| format | article |
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+, K
+-ATPase catalytic (
α) subunit in sciatic nerve of lipopolysaccharide (endotoxin, LPS)-treated rat was investigated. Using Western blot to determine subunit isoform polypeptide levels in rat sciatic nerve, we found a substantial reduction in
α1 polypeptide, but not that of
α2 and
α3 polypeptides, after the administration of LPS. Moreover, when rats were treated with polymyxin B (a LPS neutralizer) and
N
G-nitro-
l-arginine (an inhibitor of nitric oxide (NO) synthase), the effects of LPS were reversed. These results implicate a specific marked deficit in
α1 subunit isoform of Na
+,K
+-ATPase in the pathogenesis of neuropathy during endotoxemia, through, at least in part, the
l-arginine/NO pathway.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/S0304-3940(97)00686-1</identifier><identifier>PMID: 9364523</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; Anti-Bacterial Agents - pharmacology ; Binding Sites ; Biological and medical sciences ; Catalysis ; Endotoxemia - enzymology ; Endotoxin ; Enzyme Inhibitors - pharmacology ; Fundamental and applied biological sciences. Psychology ; Isoenzymes - drug effects ; Lipopolysaccharides - pharmacology ; Male ; Na +,K +-ATPase ; Nerve ; Nitric oxide ; Nitric Oxide - physiology ; Nitroarginine - pharmacology ; Peptide Fragments - drug effects ; Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ ; Polymyxin B - pharmacology ; Rats ; Rats, Wistar ; Sciatic Nerve - drug effects ; Sciatic Nerve - enzymology ; Sodium-Potassium-Exchanging ATPase - drug effects ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroscience letters, 1997-10, Vol.234 (2), p.166-168</ispartof><rights>1997 Elsevier Science Ireland Ltd</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-9985d23b7cee517233ae625212d7035c9f93a1ad06f67e99f995a79f89fd4a583</citedby><cites>FETCH-LOGICAL-c389t-9985d23b7cee517233ae625212d7035c9f93a1ad06f67e99f995a79f89fd4a583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2840644$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9364523$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Shing H</creatorcontrib><creatorcontrib>Sheu, Tzong J</creatorcontrib><title>The in vivo effect of lipopolysaccharide on Na +,K +-ATPase catalytic ( α) subunit isoforms in rat sciatic nerve</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>The Na
+, K
+-ATPase catalytic (
α) subunit in sciatic nerve of lipopolysaccharide (endotoxin, LPS)-treated rat was investigated. Using Western blot to determine subunit isoform polypeptide levels in rat sciatic nerve, we found a substantial reduction in
α1 polypeptide, but not that of
α2 and
α3 polypeptides, after the administration of LPS. Moreover, when rats were treated with polymyxin B (a LPS neutralizer) and
N
G-nitro-
l-arginine (an inhibitor of nitric oxide (NO) synthase), the effects of LPS were reversed. These results implicate a specific marked deficit in
α1 subunit isoform of Na
+,K
+-ATPase in the pathogenesis of neuropathy during endotoxemia, through, at least in part, the
l-arginine/NO pathway.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>Catalysis</subject><subject>Endotoxemia - enzymology</subject><subject>Endotoxin</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Isoenzymes - drug effects</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Male</subject><subject>Na +,K +-ATPase</subject><subject>Nerve</subject><subject>Nitric oxide</subject><subject>Nitric Oxide - physiology</subject><subject>Nitroarginine - pharmacology</subject><subject>Peptide Fragments - drug effects</subject><subject>Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ</subject><subject>Polymyxin B - pharmacology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Sciatic Nerve - drug effects</subject><subject>Sciatic Nerve - enzymology</subject><subject>Sodium-Potassium-Exchanging ATPase - drug effects</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNqFkc1qGzEQgEVpSd2kjxDQoZSEdFv9rFarUwghaUNCU6h7FmPtiKisV460a_Bj9UX6TJVj42tPwzDf_PANIaecfeaMN19-MsnqSpqanRl9zljTNhV_RWa81aLSRovXZHZA3pJ3Of9mjCmu6iNyZGRTKyFn5Hn-hDQMdB3WkaL36EYaPe3DKq5iv8ng3BOk0CGNA_0O9OLTPb2oruY_ICN1MEK_GYOjZ_Tvn3Oap8U0hJGGHH1My7wdnGCk2QXYUgOmNZ6QNx76jO_38Zj8ur2ZX3-rHh6_3l1fPVROtmasjGlVJ-RCO0TFtZASsBFKcNFpJpUz3kjg0LHGNxpNSY0CbXxrfFeDauUx-bibu0rxecI82mXIDvseBoxTtrpoUZybAqod6FLMOaG3qxSWkDaWM7tVbV9U261Ha7R9UW156TvdL5gWS-wOXXu3pf5hX4fsoPcJBhfyARNtzZq6LtjlDsMiYx0w2aILB4ddSOUbtovhP4f8A5r2mdk</recordid><startdate>19971003</startdate><enddate>19971003</enddate><creator>Liu, Shing H</creator><creator>Sheu, Tzong J</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19971003</creationdate><title>The in vivo effect of lipopolysaccharide on Na +,K +-ATPase catalytic ( α) subunit isoforms in rat sciatic nerve</title><author>Liu, Shing H ; Sheu, Tzong J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-9985d23b7cee517233ae625212d7035c9f93a1ad06f67e99f995a79f89fd4a583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Binding Sites</topic><topic>Biological and medical sciences</topic><topic>Catalysis</topic><topic>Endotoxemia - enzymology</topic><topic>Endotoxin</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Isoenzymes - drug effects</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Male</topic><topic>Na +,K +-ATPase</topic><topic>Nerve</topic><topic>Nitric oxide</topic><topic>Nitric Oxide - physiology</topic><topic>Nitroarginine - pharmacology</topic><topic>Peptide Fragments - drug effects</topic><topic>Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ</topic><topic>Polymyxin B - pharmacology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Sciatic Nerve - drug effects</topic><topic>Sciatic Nerve - enzymology</topic><topic>Sodium-Potassium-Exchanging ATPase - drug effects</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Shing H</creatorcontrib><creatorcontrib>Sheu, Tzong J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Shing H</au><au>Sheu, Tzong J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The in vivo effect of lipopolysaccharide on Na +,K +-ATPase catalytic ( α) subunit isoforms in rat sciatic nerve</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>1997-10-03</date><risdate>1997</risdate><volume>234</volume><issue>2</issue><spage>166</spage><epage>168</epage><pages>166-168</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>The Na
+, K
+-ATPase catalytic (
α) subunit in sciatic nerve of lipopolysaccharide (endotoxin, LPS)-treated rat was investigated. Using Western blot to determine subunit isoform polypeptide levels in rat sciatic nerve, we found a substantial reduction in
α1 polypeptide, but not that of
α2 and
α3 polypeptides, after the administration of LPS. Moreover, when rats were treated with polymyxin B (a LPS neutralizer) and
N
G-nitro-
l-arginine (an inhibitor of nitric oxide (NO) synthase), the effects of LPS were reversed. These results implicate a specific marked deficit in
α1 subunit isoform of Na
+,K
+-ATPase in the pathogenesis of neuropathy during endotoxemia, through, at least in part, the
l-arginine/NO pathway.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>9364523</pmid><doi>10.1016/S0304-3940(97)00686-1</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-3940 |
| ispartof | Neuroscience letters, 1997-10, Vol.234 (2), p.166-168 |
| issn | 0304-3940 1872-7972 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79405119 |
| source | ScienceDirect Journals |
| subjects | Animals Anti-Bacterial Agents - pharmacology Binding Sites Biological and medical sciences Catalysis Endotoxemia - enzymology Endotoxin Enzyme Inhibitors - pharmacology Fundamental and applied biological sciences. Psychology Isoenzymes - drug effects Lipopolysaccharides - pharmacology Male Na +,K +-ATPase Nerve Nitric oxide Nitric Oxide - physiology Nitroarginine - pharmacology Peptide Fragments - drug effects Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ Polymyxin B - pharmacology Rats Rats, Wistar Sciatic Nerve - drug effects Sciatic Nerve - enzymology Sodium-Potassium-Exchanging ATPase - drug effects Vertebrates: nervous system and sense organs |
| title | The in vivo effect of lipopolysaccharide on Na +,K +-ATPase catalytic ( α) subunit isoforms in rat sciatic nerve |
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