Transcription of dispersed repeated sequences during Pleurodeles waltl oogenesis

Summary— Several repeated DNA sequences were isolated from a partial genomic DNA library of the newt Pleurodeles waltl. These repeated DNA elements are dispersed over the 12 P. waltl bivalents, and some of them are transcribed in the oocyte. We describe the localization of label following in situ hy...

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Bibliographic Details
Published in:Biology of the cell 1989-01, Vol.67 (1), p.9-18
Main Authors: Randsholt, Neel B., Muller, Jean-Pierre, Loones, Marie-Thérèse
Format: Article
Language:English
Subjects:
Animals
Autoradiography
Chromosomes - physiology
DNA Probes
Female
in situ hybridization
lampbrush chromosomes
Nucleic Acid Hybridization
Oocytes - physiology
Oocytes - ultrastructure
Oogenesis - genetics
Pleurodeles - genetics
Pleurodeles waltl
Repetitive Sequences, Nucleic Acid - genetics
Restriction Mapping
Salamandridae - genetics
transcription of repeated sequences
Transcription, Genetic
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Summary:Summary— Several repeated DNA sequences were isolated from a partial genomic DNA library of the newt Pleurodeles waltl. These repeated DNA elements are dispersed over the 12 P. waltl bivalents, and some of them are transcribed in the oocyte. We describe the localization of label following in situ hybridization to nascent RNA attached to the lateral loops of lampbrush chromosomes. Variations in the number and the location of labelled loops were constantly found for several of the probes. The results are discussed in view of the “cotranscription model” of RNA synthesis on lampbrush chromosomes. We speculate on the possible origins of variation in transcription on lampbrush loops.
ISSN:0248-4900
1768-322X
DOI:10.1111/j.1768-322X.1989.tb03005.x