Buspirone metabolite structure profile using a standard liquid chromatographic-mass spectrometric protocol

A rapid and systematic LC-MS protocol is utilized to profile buspirone metabolites. Analysis of rat bile, urine and liver S9 samples using a standard LC-MS method provides structural information for 25 metabolites. The resulting buspirone metabolite structure database contains characteristic retenti...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 1997-09, Vol.698 (1), p.133-145
Main Authors: Kerns, Edward H., Rourick, Robyn A., Volk, Kevin J., Lee, Mike S.
Format: Article
Language:English
Subjects:
Analysis
Animals
Anti-Anxiety Agents - metabolism
Biological and medical sciences
Body Fluids - metabolism
Buspirone
Buspirone - metabolism
Chromatography, High Pressure Liquid - methods
General pharmacology
Male
Mass Spectrometry - methods
Medical sciences
Pharmacology. Drug treatments
Rats
Rats, Sprague-Dawley
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Description
Summary:A rapid and systematic LC-MS protocol is utilized to profile buspirone metabolites. Analysis of rat bile, urine and liver S9 samples using a standard LC-MS method provides structural information for 25 metabolites. The resulting buspirone metabolite structure database contains characteristic retention time, molecular mass and MS-MS product ion information for each compound. Metabolites are categorized according to profile groups, which illustrate that substitution reactions are primarily associated with the azaspirone decane dione and pyrimidine substructures. Structures of new buspirone metabolites are reported and include the despyrimidinyl, despyrimidinylpiperazine, glucuronide, hydroxyglucuronide (four isomers), methoxyglucuronide and hydroxymethoxyglucuronide (two isomers) buspirone metabolites.
ISSN:0378-4347
1387-2273
DOI:10.1016/S0378-4347(97)00254-5