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Buspirone metabolite structure profile using a standard liquid chromatographic-mass spectrometric protocol

A rapid and systematic LC-MS protocol is utilized to profile buspirone metabolites. Analysis of rat bile, urine and liver S9 samples using a standard LC-MS method provides structural information for 25 metabolites. The resulting buspirone metabolite structure database contains characteristic retenti...

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Published in:	Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 1997-09, Vol.698 (1), p.133-145
Main Authors:	Kerns, Edward H., Rourick, Robyn A., Volk, Kevin J., Lee, Mike S.
Format:	Article
Language:	English
Subjects:	Analysis Animals Anti-Anxiety Agents - metabolism Biological and medical sciences Body Fluids - metabolism Buspirone Buspirone - metabolism Chromatography, High Pressure Liquid - methods General pharmacology Male Mass Spectrometry - methods Medical sciences Pharmacology. Drug treatments Rats Rats, Sprague-Dawley
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container_title	Journal of chromatography. B, Biomedical sciences and applications
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description	A rapid and systematic LC-MS protocol is utilized to profile buspirone metabolites. Analysis of rat bile, urine and liver S9 samples using a standard LC-MS method provides structural information for 25 metabolites. The resulting buspirone metabolite structure database contains characteristic retention time, molecular mass and MS-MS product ion information for each compound. Metabolites are categorized according to profile groups, which illustrate that substitution reactions are primarily associated with the azaspirone decane dione and pyrimidine substructures. Structures of new buspirone metabolites are reported and include the despyrimidinyl, despyrimidinylpiperazine, glucuronide, hydroxyglucuronide (four isomers), methoxyglucuronide and hydroxymethoxyglucuronide (two isomers) buspirone metabolites.
doi_str_mv	10.1016/S0378-4347(97)00254-5
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source	ScienceDirect Freedom Collection 2022-2024
subjects	Analysis Animals Anti-Anxiety Agents - metabolism Biological and medical sciences Body Fluids - metabolism Buspirone Buspirone - metabolism Chromatography, High Pressure Liquid - methods General pharmacology Male Mass Spectrometry - methods Medical sciences Pharmacology. Drug treatments Rats Rats, Sprague-Dawley
title	Buspirone metabolite structure profile using a standard liquid chromatographic-mass spectrometric protocol
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