MPc2, a new murine homolog of the Drosophila polycomb protein is a member of the mouse polycomb transcriptional repressor complex

The evolutionarily conserved polycomb and trithorax-group genes are required to maintain stable expression patterns of homeotic genes and other target genes throughout development. Here, we report the cloning and characterization of a novel mouse polycomb homolog, MPc2, in addition to the previously...

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Bibliographic Details
Published in:Journal of molecular biology 1997-11, Vol.273 (5), p.993-1003
Main Authors: Alkema, M J, Jacobs, J, Voncken, J W, Jenkins, N A, Copeland, N G, Satijn, D P, Otte, A P, Berns, A, van Lohuizen, M
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Chromosome Mapping
Chromosomes, Human, Pair 17 - genetics
Consensus Sequence
Crosses, Genetic
DNA, Complementary - genetics
Drosophila melanogaster - genetics
Female
Genes
Genes, Reporter
Humans
Male
Mice - genetics
Mice, Mutant Strains
Mice, Transgenic
Molecular Sequence Data
Muridae - genetics
Nuclear Proteins - metabolism
Polycomb Repressive Complex 1
Polycomb-Group Proteins
Proto-Oncogene Proteins - metabolism
Recombinant Fusion Proteins - metabolism
Repressor Proteins - genetics
Repressor Proteins - physiology
Sequence Alignment
Sequence Homology, Amino Acid
Species Specificity
Transcription, Genetic
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Summary:The evolutionarily conserved polycomb and trithorax-group genes are required to maintain stable expression patterns of homeotic genes and other target genes throughout development. Here, we report the cloning and characterization of a novel mouse polycomb homolog, MPc2, in addition to the previously described M33 polycomb gene. Co-immunoprecipitations and subnuclear co-localization studies show that MPc2 interacts with the mouse polycomb-group oncoprotein Bmi1 and is a new member of the mouse polycomb multiprotein complex. Gal4DB-MPc2 or -M33 fusion proteins mediate a five- to tenfold repression of stably integrated reporter constructs carrying GAL4 binding sites, demonstrating that these proteins are transcriptional repressors. The MPc2 gene is localized on chromosome 11, in close proximity to the classical mouse mutations tail short (Ts) and rabo torcido (Rbt). Ts and Rbt hemizygous mice display anemia and transformations of the axial skeleton reminiscent of phenotypes observed in mice with mutated polycomb or trithorax-group genes, suggesting that MPc2 is a candidate gene for Ts and Rbt.
ISSN:0022-2836
DOI:10.1006/jmbi.1997.1372