Improved synthesis of myo-inositol 1-(4-nitrophenyl hydrogen phosphate), a chromogenic substrate for phosphatidylinositol-specific phospholipase C

This paper describes an improved procedure for the synthesis of racemic myo-inositol 1-(4-nitrophenyl hydrogen phosphate) (NPIP) a useful substrate for the continuous spectrophotometric assay of phosphatidylinositol-specific phospholipase C (PI-PLC).

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Bibliographic Details
Published in:Chemistry and physics of lipids 1997-10, Vol.89 (2), p.153-157
Main Authors: Rukavishnikov, Aleksey V., O. Zaikova, Tatiana, Griffith, O.Hayes, Keana, John F.W.
Format: Article
Language:English
Subjects:
4-Nitrophenol
Enzyme assay
Inositol Phosphates - chemical synthesis
Inositol Phosphates - chemistry
Magnetic Resonance Spectroscopy
p-Nitrophenol
Phosphatidylinositol Diacylglycerol-Lyase
Phosphoinositide Phospholipase C
Phosphoinositide-specific phospholipase C
Stereoisomerism
Substrate analog
Substrate Specificity
Type C Phospholipases - metabolism
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Description
Summary:This paper describes an improved procedure for the synthesis of racemic myo-inositol 1-(4-nitrophenyl hydrogen phosphate) (NPIP) a useful substrate for the continuous spectrophotometric assay of phosphatidylinositol-specific phospholipase C (PI-PLC).
ISSN:0009-3084
1873-2941
DOI:10.1016/S0009-3084(97)00069-8