In vitro holding and PLD-repair: II. A flow cytometric and electron microscopic analysis of some mammalian cell lines

The repair of potentially lethal damage (PLDR) in mammalian cells is expected to be better in quiescent cultures since PLD is supposedly fixed during cycle progression. Plateau phase cultures, therefore, serve as models because of assumed mitotic quiescence. Four established cell lines (V79, CHO, L5...

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Bibliographic Details
Published in:Radiation and environmental biophysics 1989-12, Vol.28 (4), p.277-290
Main Authors: Stevenson, A F, Palackal, T, Lange, C S
Format: Article
Language:English
Subjects:
Animals
Cell Fractionation
Cell Line
Cell Survival - radiation effects
Colony-Forming Units Assay
Cricetinae
Cricetulus
Culture Media
DNA Repair
Flow Cytometry
HeLa Cells
Humans
Mice
Microscopy, Electron
Mitosis - genetics
Tumor Cells, Cultured
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Summary:The repair of potentially lethal damage (PLDR) in mammalian cells is expected to be better in quiescent cultures since PLD is supposedly fixed during cycle progression. Plateau phase cultures, therefore, serve as models because of assumed mitotic quiescence. Four established cell lines (V79, CHO, L5178Y and HELA) and one euploid cell strain IMR-90 have been analysed by flow cytometry and electron microscopy to address questions on quiescence in the plateau phase and the effect of holding (induction of quiescence by nutrient privation). In contrast to commonly held views, our results indicate that the quiescent fraction in cultures from transformed cells is exceedingly low (1% or less). Plateau phase cultures of transformed cells are constantly turning over. Euploid cells like the IMR-90 show true quiescence in the plateau phase. Holding causes typical cytopathological changes. These changes have been ultrastructurally++ characterised. Resistant sub-populations of cells can be selected out under holding-conditions. Such selected cells show completely different radiobiological characteristics, which raise questions on the interpretation of data on PLDR.
ISSN:0301-634X
1432-2099
DOI:10.1007/BF01212968