In vitro holding and PLD-repair: II. A flow cytometric and electron microscopic analysis of some mammalian cell lines

• Published in: Radiation and environmental biophysics 1989-12, Vol.28 (4), p.277-290
• Main Authors: Stevenson, A F, Palackal, T, Lange, C S
• Format: Article
• Language: English
• Subjects: Animals; Cell Fractionation; Cell Line; Cell Survival - radiation effects; Colony-Forming Units Assay; Cricetinae; Cricetulus; Culture Media; DNA Repair; Flow Cytometry; HeLa Cells; Humans; Mice; Microscopy, Electron; Mitosis - genetics; Tumor Cells, Cultured
• ISSN: 0301-634X; 1432-2099
• DOI: 10.1007/BF01212968

The repair of potentially lethal damage (PLDR) in mammalian cells is expected to be better in quiescent cultures since PLD is supposedly fixed during cycle progression. Plateau phase cultures, therefore, serve as models because of assumed mitotic quiescence. Four established cell lines (V79, CHO, L5178Y and HELA) and one euploid cell strain IMR-90 have been analysed by flow cytometry and electron microscopy to address questions on quiescence in the plateau phase and the effect of holding (induction of quiescence by nutrient privation). In contrast to commonly held views, our results indicate that the quiescent fraction in cultures from transformed cells is exceedingly low (1% or less). Plateau phase cultures of transformed cells are constantly turning over. Euploid cells like the IMR-90 show true quiescence in the plateau phase. Holding causes typical cytopathological changes. These changes have been ultrastructurally++ characterised. Resistant sub-populations of cells can be selected out under holding-conditions. Such selected cells show completely different radiobiological characteristics, which raise questions on the interpretation of data on PLDR.