Culture and Characterization of Fetal Human Atrial and Ventricular Cardiac Muscle Cells

Atrial and ventricular cardiac muscle cells isolated from 14- to 18-wk old fetal human hearts were grown in culture and characterized. Once established in culture the flattened cells contracted spontaneously and possessed differentiated ultrastructural characteristics including organized sarcomeres,...

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Bibliographic Details
Published in:In Vitro Cellular & Developmental Biology 1989-12, Vol.25 (12), p.1114-1120
Main Authors: Claycomb, William C., Delcarpio, Joseph B., Guice, Sally E., Moses, R. L.
Format: Article
Language:English
Subjects:
Adults
Animal cells
Atrial Natriuretic Factor - genetics
Biological and medical sciences
Biotechnology
Blotting, Northern
Cell culture techniques
Cell cultures. Hybridization. Fusion
Cell separation
Cells, Cultured
Cellular differentiation
Cultured cells
Establishment of new cell lines, improvement of cultural methods, mass cultures
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Glycogen
Heart
Heart Atria - cytology
Heart Atria - embryology
Heart Ventricles - cytology
Heart Ventricles - embryology
Humans
Insulin-Like Growth Factor II - genetics
Methods. Procedures. Technologies
Microscopy, Electron
Microscopy, Electron, Scanning
Molecular and cellular biology
Muscle Proteins - genetics
Muscle tissues
Myocardial Contraction
Myocardium
RNA, Messenger - genetics
Tissue culture techniques
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Summary:Atrial and ventricular cardiac muscle cells isolated from 14- to 18-wk old fetal human hearts were grown in culture and characterized. Once established in culture the flattened cells contracted spontaneously and possessed differentiated ultrastructural characteristics including organized sarcomeres, intercalated discs, and transverse tubules with couplings. Atrial granules were present in the cultured atrial cells. Some cultured ventricular myocytes also contained electron-dense granules associated with Golgi cisternae, which were similar in size and appearance to atrial granules. The cultured ventricular myocytes divided and expressed the genes for thymidine kinase, histone H4, myosin heavy chain, muscle-specific creatine kinase, atrial natriuretic factor, and insulin-like growth factor II. These results establish that differentiated fetal human heart muscle cells can be cultured in sufficient quantities for biochemical, molecular, and morphological analyses.
ISSN:0883-8364
2327-431X
1475-2689
DOI:10.1007/BF02621262