Strain differentiation of isolates of streptococci from bovine mastitis by pulsed-field gel electrophoresis

Pulsed-field gel electrophoresis (PFGE) was examined as a tool to differentiate strains of streptococci isolated from clinical and sub-clinical cases of bovine mastitis. Analysis ofSmaI chromosomal digests ofStreptococcus agalactiae,S. dysgalactiaesubsp.dysgalactiaeandS. uberisisolates revealed intr...

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Bibliographic Details
Published in:Molecular and cellular probes 1997-10, Vol.11 (5), p.349-354
Main Authors: Baseggio, Nina, Mansell, Peter D., Browning, Jeff W., Browning, Glenn F.
Format: Article
Language:English
Subjects:
Animals
Bacterial Typing Techniques - veterinary
Cattle
Deoxyribonucleases, Type II Site-Specific - metabolism
DNA, Bacterial - analysis
Electrophoresis, Gel, Pulsed-Field
epidemiology
Female
Mastitis, Bovine - microbiology
strain typing
Streptococcal Infections - microbiology
Streptococcal Infections - veterinary
Streptococcus - classification
Streptococcus - genetics
Streptococcus - isolation & purification
Streptococcus agalactiae
Streptococcus dysgalactiaesubspeciesdysgalactiae
Streptococcus uberis
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Summary:Pulsed-field gel electrophoresis (PFGE) was examined as a tool to differentiate strains of streptococci isolated from clinical and sub-clinical cases of bovine mastitis. Analysis ofSmaI chromosomal digests ofStreptococcus agalactiae,S. dysgalactiaesubsp.dysgalactiaeandS. uberisisolates revealed intraherd and interherd strain relationships within each species. Comparison ofS. agalactiaeisolates from the same herd revealed little variability in theirSmaI restriction patterns indicating a single strain originating from a common source of infection. However, comparison ofS. agalactiaeisolates between herds showed that each herd was infected with a distinct strain. The restriction profiles fromS. dysgalactiaesubsp.dysgalactiaeandS. uberiswere more diverse and often multiple strains of both species were present within an individual herd, with some herds containing more than one representative of a particular strain. Interestingly, it was only observed withS. dysgalactiaesubsp.dysgalactiaethat some strains were present in more than one herd. PFGE was found to be a useful and reproducible method for the discrimination of different strains of the three most important species of streptococci responsible for bovine mastitis and to offer a means to identify environmental sources of infection.
ISSN:0890-8508
1096-1194
DOI:10.1006/mcpr.1997.0126