Tandem-in-Time Mass Spectrometry as a Quantitative Bioanalytical Tool

Tandem-in-time mass spectrometry, as implemented on an ion-trap detector (ITD), is the process whereby precursor ions are created, stored in a radio frequency (rf) trapping field, and then sequentially fragmented to form product ions by application of additional rf waveforms. As with any form of tan...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 1997-11, Vol.69 (22), p.4519-4523
Main Authors: Rossi, David T, Hoffman, Keith L, Janiczek-Dolphin, Nancy, Bockbrader, Howard, Parker, Tresavon D
Format: Article
Language:English
Subjects:
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Bioassay
Biochemistry
Biological and medical sciences
Biotechnology
Characterization
Cholinesterase Inhibitors - blood
Fundamental and applied biological sciences. Psychology
Gas chromatography
Gas Chromatography-Mass Spectrometry
Humans
Ion exchange
Ionization
Ions
Mass Spectrometry - methods
Phase composition
Scientific imaging
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Summary:Tandem-in-time mass spectrometry, as implemented on an ion-trap detector (ITD), is the process whereby precursor ions are created, stored in a radio frequency (rf) trapping field, and then sequentially fragmented to form product ions by application of additional rf waveforms. As with any form of tandem mass spectrometry (MS/MS), tandem-in-time MS is highly selective, by virtue of both mass discrimination and specific gas-phase chemistry. Beyond this, however, tandem-in-time MS offers ion throughput efficiency and cost advantages over either quadrupole or sector instruments. This paper will describe the use of capillary gas chromatography combined with tandem-in-time mass spectrometry to quantify a novel therapeutic agent extracted from human plasma. For an example compound, a quantitation limit of 25 pg/mL (S/N ≈ 10, 15 fmol on-column) was attained out of plasma. The interday imprecision was ≤12.2% over a dynamic range extending to 10 ng/mL. Due to favorable ionization conditions for the test analytes, electron ionization resulted in formation of M+ ions, with very little fragmentation, allowing for maximum assay sensitivity. Although method characterization and validation demonstrated adequate instrumental performance, some lack of ruggedness was encountered during routine application.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac970247n