Deletion of a consensus oestrogen response element half‐site in the glucocorticoid receptor of human multiple myeloma

We have carried out molecular scanning of the glucocorticoid receptor (GR) of the glucocorticoid resistant multiple myeloma cell line U266. An amplified fragment from the 3′ untranslated region displayed an aberrant migration by PCR–single‐stranded conformational polymorphism (PCR‐SSCP) analysis. Th...

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Bibliographic Details
Published in:British journal of haematology 1997-11, Vol.99 (2), p.372-374
Main Authors: Karkera, Jayaprakash D., Taymans, Susan E., Turner, Gordon, Yoshikawa, Takeo, Detera‐Wadleigh, Sevilla D., Wadleigh, Robert G.
Format: Article
Language:English
Subjects:
3′ untranslated region
Antineoplastic agents
Biological and medical sciences
deletion
Estrogens - genetics
exon 9α
Exons
General aspects
glucocorticoid resistance
Humans
Medical sciences
Multiple Myeloma - genetics
Mutation
PCR‐SSCP
Pharmacology. Drug treatments
Polymerase Chain Reaction
Receptors, Glucocorticoid - genetics
Sequence Deletion
Tumor Cells, Cultured
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Summary:We have carried out molecular scanning of the glucocorticoid receptor (GR) of the glucocorticoid resistant multiple myeloma cell line U266. An amplified fragment from the 3′ untranslated region displayed an aberrant migration by PCR–single‐stranded conformational polymorphism (PCR‐SSCP) analysis. The mutant allele had a deletion of an 8 base pair sequence containing a half‐site of an oestrogen response element. This motif was found conserved in rat GR. This same allele lacked four As in an upstream region with 18 consecutive As in the normal allele. These mutations may affect mRNA stability or alter interactions with regulatory factors.
ISSN:0007-1048
1365-2141
DOI:10.1046/j.1365-2141.1997.4123227.x