Characterization of an N-acetylglucosamine-6-O-sulfotransferase from human respiratory mucosa active on mucin carbohydrate chains

A microsomal GlcNAc-6-O-sulfotransferase activity from human bronchial mucosa, able to transfer a sulfate group from adenosine 3'-phosphate 5'-phosphosulfate onto methyl-N-acetylglucosaminides or terminal N-acetylglucosamine residues of carbohydrate chains from human respiratory mucins, ha...

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Bibliographic Details
Published in:The Journal of biological chemistry 1997-11, Vol.272 (47), p.29493-29501
Main Authors: Degroote, S, Lo-Guidice, J M, Strecker, G, Ducourouble, M P, Roussel, P, Lamblin, G
Format: Article
Language:English
Subjects:
Acetylglucosamine - metabolism
Binding, Competitive
Bronchi - enzymology
Carbohydrate Conformation
Carbohydrate Sulfotransferases
Chromatography, High Pressure Liquid
Galactose - metabolism
Galactosyltransferases - metabolism
Humans
Hydrogen-Ion Concentration
Microsomes - enzymology
Molecular Sequence Data
Mucins - metabolism
Mucous Membrane - enzymology
Phosphoadenosine Phosphosulfate - metabolism
Sulfates - metabolism
Sulfotransferases - chemistry
Sulfotransferases - metabolism
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Summary:A microsomal GlcNAc-6-O-sulfotransferase activity from human bronchial mucosa, able to transfer a sulfate group from adenosine 3'-phosphate 5'-phosphosulfate onto methyl-N-acetylglucosaminides or terminal N-acetylglucosamine residues of carbohydrate chains from human respiratory mucins, has been characterized. The reaction products containing a terminal HO3S-6GlcNAc were identified by high performance anion-exchange chromatography. Using methyl-beta-N-acetylglucosaminide as a substrate, the optimal activity was obtained with 0.1% Triton X-100, 30 mM NaF, 20 mM Mn2+, 5 mM AMP in a 30 mM MOPS (3-(N-morpholino) propanesulfonic acid) buffer at pH 6.7. The apparent Km values for adenosine 3'-phosphate 5'-phosphosulfate and methyl-beta-N-acetylglucosaminide were observed at 9.1 x 10(-6) M and 0.54 x 10(-3) M, respectively. The enzyme had more affinity for carbohydrate chains with a terminal GlcNAc residue than for methyl-beta-N-acetylglucosaminide; it was unable to catalyze the transfer of sulfate to position 6 of the GlcNAc residue contained in a terminal Galbeta1-4GlcNAc sequence. However, oligosaccharides with a nonreducing terminal HO3S-6GlcNAc were substrates for a beta1-4 galactosyltransferase from human bronchial mucosa. These data point out that GlcNAc-6-O-sulfotransferase must act before beta1-4 galactosylation in mucin-type oligosaccharide biosynthesis.
ISSN:0021-9258
DOI:10.1074/jbc.272.47.29493