The maximum and minimum water content and cell volume of human erythrocytes in vitro

The maximum and minimum water contents of human erythrocytes were measured after exposure to various osmotic pressures. Within a range of osmolarities, at which no haemolysis occurred, the water content reached its maximum, 78.1%, at 180 mosM and its minimum, 54.8%, at 800 mosM. Simultaneously, the...

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Bibliographic Details
Published in:Biophysical chemistry 1989-09, Vol.34 (1), p.79-82
Main Authors: Kageyama, K., Onoyama, Y., Kogawa, H., Goto, E., Tanabe, K.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell morphology
Cell volume
Erythrocyte
Erythrocyte Volume
Erythrocytes - analysis
Fundamental and applied biological sciences. Psychology
Gas-liquid chromatography
Hemolysis
Human
Humans
Hydrogen-Ion Concentration
Male
Molecular and cellular biology
Osmolar Concentration
Osmolarity
Osmotic Pressure
Water - analysis
Water content
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Summary:The maximum and minimum water contents of human erythrocytes were measured after exposure to various osmotic pressures. Within a range of osmolarities, at which no haemolysis occurred, the water content reached its maximum, 78.1%, at 180 mosM and its minimum, 54.8%, at 800 mosM. Simultaneously, the mean cell volume increased to 98.5 μm 3 at 180 mosM and decreased to 77.2 μm 3 at 800 mosM.
ISSN:0301-4622
1873-4200
DOI:10.1016/0301-4622(89)80044-4