DNA sequence analysis of the apocytochrome b gene of Podospora anserina: a new family of intronic open reading frame

The 5,969 bp (base pair) DNA sequence of the apocytochrome b mitochondrial (mt) gene of race A Podospora anserina was located in a 8.5 Kbp region. This gene contained a 2,499 bp subgroup IB and a 1,306 bp subgroup ID intron as well as a 990 bp subgroup IB intron which is present in race A but not ra...

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Bibliographic Details
Published in:Current genetics 1989-12, Vol.16 (5/6), p.407-418
Main Authors: Cummings, D.J, Michel, F, McNally, K.L
Format: Article
Language:English
Subjects:
Amino Acid Sequence
apocytochrome b
Apoproteins - genetics
Ascomycota - enzymology
Ascomycota - genetics
Base Sequence
Biological and medical sciences
Cytochrome b Group - genetics
cytochromes
Cytochromes b
DNA
DNA, Fungal - genetics
DNA, Mitochondrial - genetics
Exons
Fundamental and applied biological sciences. Psychology
fungi
genes
Genes. Genome
Introns
mitochondria
Mitochondria - enzymology
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Nucleic Acid Conformation
nucleotide sequences
Podospora anserina
Sequence Homology, Nucleic Acid
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Summary:The 5,969 bp (base pair) DNA sequence of the apocytochrome b mitochondrial (mt) gene of race A Podospora anserina was located in a 8.5 Kbp region. This gene contained a 2,499 bp subgroup IB and a 1,306 bp subgroup ID intron as well as a 990 bp subgroup IB intron which is present in race A but not race s. The large subgroup IB intron and the race A specific IB intron both contained potential alternate splice sites which brought their open reading frames into phase with their upstream exon sequences. All three introns were compared with regard to their secondary structures and open reading frames to the other 30 group I introns in Podospora anserina, as well as to other fungal introns. We detected a new family of intronic ORFs comprising seven P. anserina introns, several N. crassa introns, as well as the T4td bacteriophage intron. Sequence similarities to intron-encoded endonucleases were noteworthy. The DNA sequences reported here and in the accompanying paper complete the analysis of race s and race A mitochondrial DNA.
ISSN:0172-8083
1432-0983
DOI:10.1007/BF00340720