Cryopreservation of Aspergillus fumigatus stock cultures with a commercial bead system

A method using beads and storage at — 80 °C was used to maintain isolates of the pathogenic mould Aspergillus fumigatus. Viability was assessed after 6 and 15 months and all isolates were recovered in culture. This inexpensive technique was found to be an effective and easy method of preserving A. f...

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Bibliographic Details
Published in:Mycoses 1997-09, Vol.40 (3-4), p.103-104
Main Authors: Belkacemi, L., Barton, R. C., Evans, E. G. V.
Format: Article
Language:English
Subjects:
Aspergillus fumigatus
Aspergillus fumigatus - growth & development
Aspergillus fumigatus - isolation & purification
Biological and medical sciences
cryopreservation
Cryopreservation - methods
Fundamental and applied biological sciences. Psychology
Kryokonservierung
Microbiological Techniques
Microbiology
Miscellaneous
Mycology
polystyrene beads
Polystyrolperlen
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Summary:A method using beads and storage at — 80 °C was used to maintain isolates of the pathogenic mould Aspergillus fumigatus. Viability was assessed after 6 and 15 months and all isolates were recovered in culture. This inexpensive technique was found to be an effective and easy method of preserving A. fumigatus and other pathogenic moulds. Zusammenfassung. Eine neue Methode zur Stammhaltung von Isolaten des pathogenen Schimmelpilzes Aspergillus fumigatus basierend auf der Verwendung von Polystyrol‐Perlen und Lagerung bei — 80 °C wird vorgestellt. Die Überlebensrate der Kulturen wurde nach 6 und 15 Monaten Lagerung bestimmt und betrug 100%. Die vorgestellte Methode stellt eine kostengünstige, effektive und einfache Technik zur Stammhaltung von A. fumigatus und anderen pathogenen Schimmelpilzen dar.
ISSN:0933-7407
1439-0507
DOI:10.1111/j.1439-0507.1997.tb00196.x