Immunoelectron microscopy of Bacillus subtilis cells secreting human interferon alpha 1 or staphylokinase

Post-embedding labelling techniques with colloidal gold-IgG or -protein A complexes were used to determine the subcellular location of IFN alpha 1 and staphylokinase secreted from Bacillus subtilis GB500 cells. Both proteins were present in the cytoplasma and the cell envelope pointing to a posttran...

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Bibliographic Details
Published in:FEMS microbiology letters 1989-12, Vol.53 (3), p.327-332
Main Authors: Wagner, B, Wagner, M, Wollweber, L, Behnke, D
Format: Article
Language:English
Subjects:
Bacillus subtilis - enzymology
Bacillus subtilis - metabolism
Bacillus subtilis - ultrastructure
Biological Transport
Humans
Immunohistochemistry
Interferon Type I - metabolism
Metalloendopeptidases - metabolism
Microscopy, Electron
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Summary:Post-embedding labelling techniques with colloidal gold-IgG or -protein A complexes were used to determine the subcellular location of IFN alpha 1 and staphylokinase secreted from Bacillus subtilis GB500 cells. Both proteins were present in the cytoplasma and the cell envelope pointing to a posttranslational mode of translocation across the cytoplasmic membrane. 5- to 10-fold higher concentrations of gold particles per 0.1 micron 2 were found on the cell envelope and clustering was observed suggesting preferential regions for secretion sites. Several control experiments ensured the specificity of the labelling data.
ISSN:0378-1097