Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions

Allelic loss of chromosome 9p21 is common in small cell lung cancer (SCLC), but inactivation of the tumor suppressor gene CDKN2a is rare, implying the existence of another target gene at 9p21. A recent deletion mapping study of chromosome 9p has also identified a site of deletion in non-small cell l...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1997-12, Vol.57 (23), p.5356-5359
Main Authors: CAIRNS, P, OKAMI, K, KING, P, BONACUM, J, AHRENDT, S, WU, L, MAO, L, JEN, J, SIDRANSKY, D
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Carcinoma, Non-Small-Cell Lung - genetics
Carcinoma, Small Cell - genetics
Chromosome Banding
Chromosome Deletion
Chromosome Mapping
Chromosomes, Human, Pair 9
DNA Mutational Analysis
DNA Probes
ELAV Proteins
ELAV-Like Protein 2
Exons
Genes, Tumor Suppressor
Genetic Markers
Humans
Introns
Lung Neoplasms - genetics
Medical sciences
Molecular Sequence Data
Nerve Tissue Proteins - genetics
Pneumology
Polymerase Chain Reaction
RNA-Binding Proteins - genetics
Tumors of the respiratory system and mediastinum
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Summary:Allelic loss of chromosome 9p21 is common in small cell lung cancer (SCLC), but inactivation of the tumor suppressor gene CDKN2a is rare, implying the existence of another target gene at 9p21. A recent deletion mapping study of chromosome 9p has also identified a site of deletion in non-small cell lung cancer (NSCLC) centered around D9S126. The Hel-N1 (human elav-like neuronal protein 1) gene encodes a neural-specific RNA binding protein that is expressed in SCLC. We have mapped this potentially important gene in lung tumorigenesis to within 100 kb of the D9S126 marker at chromosome band 9p21 by using homozygously deleted tumor cell lines and fluorescence in situ hybridization to normal metaphase spreads. Hel-N1 is, therefore, a candidate target suppressor gene in both SCLC and NSCLC. We have determined the genomic organization and intron/exon boundaries of Hel-N1 and have screened the entire coding region for mutations by sequencing 14 primary SCLCs and cell lines and 21 primary NSCLCs preselected for localized 9p21 deletion or monosomy of chromosome 9. A homozygous deletion including Hel-N1 and CDKN2a was found in a SCLC cell line, and a single-base polymorphism in exon 2 of Hel-N1 was observed in eight tumors. No somatic mutations of Hel-N1 were found in this panel of lung tumors. Hel-N1 does not appear to be a primary inactivation target of 9p21 deletion in lung cancer.
ISSN:0008-5472
1538-7445