CBP/p300 Functions as a Possible Transcriptional Coactivator of Ah Receptor Nuclear Translocator (Arnt)

A heterodimer of AhR (aryl hydrocarbon receptor) and Arnt (AhR nuclear translocator) conveys a transactivation signal of aromatic hydrocarbons such as 2,3,7,8-tetrachloro-dibenzo-p-dioxin and 3-methylcholanthrene to the genes for a group of drug-metabolizing enzymes. This inducible expression of the...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1997-10, Vol.122 (4), p.703-710
Main Authors: Kobayashi, Akira, Numayama-Tsuruta, Keiko, Sogawa, Kazuhiro, Fujii-Kuriyama, Yoshiaki
Format: Article
Language:English
Subjects:
Acetyltransferases - genetics
Acetyltransferases - metabolism
Aryl Hydrocarbon Receptor Nuclear Translocator
Cell Cycle Proteins - genetics
Cell Cycle Proteins - metabolism
Cell Line
Cloning, Molecular
coactivator
DNA-Binding Proteins
drug-inducible expression
heterodimer
Histone Acetyltransferases
p300-CBP Transcription Factors
Plasmids
Receptors, Aryl Hydrocarbon - metabolism
Saccharomyces cerevisiae - genetics
Trans-Activators - metabolism
Transcription Factors - genetics
Transcription Factors - metabolism
transcriptional activation
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Summary:A heterodimer of AhR (aryl hydrocarbon receptor) and Arnt (AhR nuclear translocator) conveys a transactivation signal of aromatic hydrocarbons such as 2,3,7,8-tetrachloro-dibenzo-p-dioxin and 3-methylcholanthrene to the genes for a group of drug-metabolizing enzymes. This inducible expression of the genes is inhibited by adenovirus E1A, suggesting that CBP/p300 is somehow involved in the transactivation of the genes by the AhR and Arnt heterodimer. Yeast and mammalian two hybrid systems revealed that CBP/p300 interacted with the transactivation domain of Arnt, but not with that of AhR, via the CREB-binding domain. The pull down assay using GST-Arnt hybrid protein confirmed the interaction between Arnt and CBP/p300. Considering these results and that Arnt or Arnt2 functions as a common partner in the formation of transcriptional regulators with other bHLH/PAS proteins such as AhR, HLF, and HIF-1α, the possibility arises that CBP/p300 is extensively involved as a coactivator in the transactivation process by bHLH/PAS (a conserved sequence motif among Per, Arnt, and Sim) heterodimer transcription factors through interaction with Arnt or Arnt2.
ISSN:0021-924X
DOI:10.1093/oxfordjournals.jbchem.a021812