Expression of glycoprotein gp43 in stage-specific forms and during dimorphic differentiation of Paracoccidioides brasiliensis

Expression of the 43 kDa glycoprotein (gp43) was analysed in several Paracoccidioides brasiliensis isolates. Using one- and two-dimensional analysis of crude cellular extracts, it was shown that protein expression in yeast and mycelium was dependent on the isolate analysed. In two strains, in both y...

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Bibliographic Details
Published in:Journal of medical and veterinary mycology 1997-09, Vol.35 (5), p.341-345
Main Authors: Mattar-Filho, R., Azevedo, M.O., Pereira, M., Jesuino, R.S.A., Salem-Izacc, S.M., Brito, W.A., Gesztesi, J.L., Soares, R.B.A., Felipe, M.S.S., Soares, C.M.A.
Format: Article
Language:English
Subjects:
Antigens, Fungal - biosynthesis
Biological and medical sciences
Blotting, Western
Electrophoresis, Gel, Two-Dimensional
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Fungal Proteins
Glycoproteins - biosynthesis
Glycoproteins - isolation & purification
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Humans
Microbiology
Molecular Weight
Mycology
Oligosaccharides - biosynthesis
Oligosaccharides - isolation & purification
Paracoccidioides - growth & development
Paracoccidioides - isolation & purification
Paracoccidioides - physiology
Paracoccidioides brasiliensis
Paracoccidioidomycosis - microbiology
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Summary:Expression of the 43 kDa glycoprotein (gp43) was analysed in several Paracoccidioides brasiliensis isolates. Using one- and two-dimensional analysis of crude cellular extracts, it was shown that protein expression in yeast and mycelium was dependent on the isolate analysed. In two strains, in both yeast and mycelium cells, gp43 was present, whereas expression was restricted to the yeast phase of two other strains. The clinical implications of this phase-specific gp43 expression are uncertain.
ISSN:1369-3786
0268-1218
1460-2709
1365-280X
DOI:10.1080/02681219780001391