Synthesis of Asymmetrically Labeled Sucrose by a Recombinant Sucrose Synthase

About 80% of radioactivity was recovered in asymmetrically labeled sucrose from UDP-[ 14 C]glucose or [ 14 C]fructose with recombinant mung bean sucrose synthase expressed in Escherichia coli harboring pEB-01. This high recovery is due to the fact that the enzyme conserving the activity of sucrose s...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1997, Vol.61 (11), p.1955-1956
Main Authors: Nakai, Tomonori, Tonouchi, Naoto, Tsuchida, Takayasu, Mori, Hitoshi, Sakai, Fukumi, Hayashi, Takahisa
Format: Article
Language:English
Subjects:
asymmetrically labeling
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Carbon Radioisotopes - chemistry
Escherichia coli - enzymology
Escherichia coli - genetics
Fructose - chemistry
Fructose - metabolism
Fundamental and applied biological sciences. Psychology
Glucosyltransferases - genetics
Glucosyltransferases - metabolism
Isotope Labeling - methods
Methods. Procedures. Technologies
mung bean
recombinant enzyme
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
sucrose
Sucrose - chemical synthesis
Sucrose - metabolism
Uridine Diphosphate Glucose - chemistry
Uridine Diphosphate Glucose - metabolism
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Summary:About 80% of radioactivity was recovered in asymmetrically labeled sucrose from UDP-[ 14 C]glucose or [ 14 C]fructose with recombinant mung bean sucrose synthase expressed in Escherichia coli harboring pEB-01. This high recovery is due to the fact that the enzyme conserving the activity of sucrose synthase has a similar affinity for UDP-glucose and fructose to an intact enzyme from the mung bean, but a lower affinity for sucrose.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.61.1955