Genetic linkage study of a major susceptibility locus (D2S125) in a British population of non-insulin dependent diabetic sib-pairs using a simple non-isotopic screening method

• Published in: Human genetics 1997-12, Vol.101 (2), p.212-213
• Main Authors: THOMAS, A. W, SHERRATT, E. J, GAGG, J. W, DAVIES, S. A, MAJID, A, ALCOLADO, J. C
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Diabetes Mellitus, Type 2 - epidemiology; Diabetes Mellitus, Type 2 - genetics; Diabetes. Impaired glucose tolerance; Disease Susceptibility; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Etiopathogenesis. Screening. Investigations. Target tissue resistance; Genetic Linkage; Genetic Testing - methods; Humans; Medical sciences; Microsatellite Repeats; Nuclear Family; United Kingdom - epidemiology
• ISSN: 0340-6717; 1432-1203
• DOI: 10.1007/s004390050617

A polymorphic microsatellite marker (D2S125) was recently reported to show significant linkage to non-insulin dependent diabetes mellitus (NIDDM) in a population of Mexican-American affected sib-pairs. We have used a simple non-isotopic screening technique employing the polymerase chain reaction (PCR) with a biotinylated primer to study the genetic linkage and allele frequency distribution of the D2S125 marker in a population of 109 British NIDDMs (62 possible affected sib-pairs). The analysis provided no evidence for linkage of the D2S125 marker in the British subjects (MLS = 0.029, P > 0.05). The PCR screening method used proved to be a convenient and reliable alternative to the radiolabelling of PCR products.