Alignment of Conduits for the Nascent Polypeptide Chain in the Ribosome-Sec61 Complex

An oligomer of the Sec61 trimeric complex is thought to form the protein-conducting channel for protein transport across the endoplasmic reticulum. A purified yeast Sec61 complex bound to monomeric yeast ribosomes as an oligomer in a saturable fashion. Cryo-electron microscopy of the ribosome-Sec61...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1997-12, Vol.278 (5346), p.2123-2126
Main Authors: Beckmann, Roland, Bubeck, Doryen, Grassucci, Robert, Penczek, Pawel, Verschoor, Adriana, Blobel, Gunter, Frank, Joachim
Format: Article
Language:English
Subjects:
Antibodies
Binding Sites
Biological and medical sciences
Biological Transport
Cells
Cellular biology
Centrifugation
Electron microscopy
Endoplasmic reticulum
Endoplasmic Reticulum, Rough - metabolism
Fundamental and applied biological sciences. Psychology
Fungal Proteins - chemistry
Fungal Proteins - metabolism
Fungal Proteins - ultrastructure
Image Processing, Computer-Assisted
Macromolecular Substances
Mammals
Membrane Proteins - chemistry
Membrane Proteins - metabolism
Membrane Proteins - ultrastructure
Membrane Transport Proteins
Microscopy
Microscopy, Electron
Molecular and cellular biology
Molecular genetics
Oligomers
Peptides
Physiological aspects
Protein biosynthesis
Protein synthesis
Protein transport
Proteins
Ribosomes
Ribosomes - chemistry
Ribosomes - metabolism
Ribosomes - ultrastructure
Saccharomyces cerevisiae - chemistry
Saccharomyces cerevisiae - ultrastructure
Saccharomyces cerevisiae Proteins
Scientific Concepts
SEC Translocation Channels
Synthesis
Translation. Translation factors. Protein processing
Tunnels
Yeast
Yeast (Food product)
Yeasts
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Description
Summary:An oligomer of the Sec61 trimeric complex is thought to form the protein-conducting channel for protein transport across the endoplasmic reticulum. A purified yeast Sec61 complex bound to monomeric yeast ribosomes as an oligomer in a saturable fashion. Cryo-electron microscopy of the ribosome-Sec61 complex and a three-dimensional reconstruction showed that the Sec61 oligomer is attached to the large ribosomal subunit by a single connection. Moreover, a funnel-shaped pore in the Sec61 oligomer aligned with the exit of a tunnel traversing the large ribosomal subunit, strongly suggesting that both structures function together in the translocation of proteins across the endoplasmic reticulum membrane.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.278.5346.2123