GDNF Induces Branching and Increased Cell Proliferation in the Ureter of the Mouse

The secreted signaling molecule GDNF is expressed in the metanephric mesenchyme and has recently been implicated as a factor necessary for development of the metanephric kidney. We have examined the effects of GDNF on mouse kidney explants. We show that GDNF increases cell proliferation in ureter ti...

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Bibliographic Details
Published in:Developmental biology 1997-12, Vol.192 (1), p.193-198
Main Authors: Pepicelli, Carmen V., Kispert, Andreas, Rowitch, David H., McMahon, Andrew P.
Format: Article
Language:English
Subjects:
Animals
Cell Division - drug effects
Female
Gene Expression Regulation, Developmental
Glial Cell Line-Derived Neurotrophic Factor
Glycoproteins - biosynthesis
Glycoproteins - genetics
Lac Operon
Male
Mice
Mice, Inbred C57BL
Mice, Inbred CBA
Mice, Transgenic
Models, Biological
Nerve Growth Factors - pharmacology
Nerve Tissue Proteins - pharmacology
Organ Culture Techniques
Up-Regulation
Ureter - cytology
Ureter - drug effects
Ureter - embryology
Wnt Proteins
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Summary:The secreted signaling molecule GDNF is expressed in the metanephric mesenchyme and has recently been implicated as a factor necessary for development of the metanephric kidney. We have examined the effects of GDNF on mouse kidney explants. We show that GDNF increases cell proliferation in ureter tips. There is an increase in the number of ureter tips and expansion and fusion of adjacent tips and some tips appear to grow toward the source of GDNF. These events are accompanied by transcriptional upregulation of several genes localized to the tips, including its own receptor,c-ret,the transcription factorSox9,and the signalWnt-11.These results support a model in which GDNF supplied by the mesenchyme regulates growth and branching in the metanephric kidney through the local regulation of ureter tip-specific factors.
ISSN:0012-1606
1095-564X
DOI:10.1006/dbio.1997.8745