Isolation of a measles virus variant: protection of newborn mice from measles encephalitis by 24 h prior intracerebral inoculation with the variant

A small plaque mutant with reduced neurovirulence in newborn mice was obtained from Edmonston strain measles virus after propagation for 5 months in NIH3T3 cells. It retained the antigenicity of the parental virus and tended to induce higher neutralizing antibody titers in the adult BALB/c mice. The...

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Bibliographic Details
Published in:Archives of virology 1997, Vol.142 (10), p.1937-1952
Main Authors: Sakae, H, Kohase, M, Kurata, T, Yoshikura, H
Format: Article
Language:English
Subjects:
3T3 Cells
Animals
Animals, Newborn
Brain - metabolism
Brain - virology
Cardiovirus Infections - mortality
Cardiovirus Infections - prevention & control
Cercopithecus aethiops
Encephalitis, Viral - prevention & control
Encephalomyocarditis virus
Female
Interferons - metabolism
Kinetics
Measles - prevention & control
Measles virus - genetics
Measles virus - immunology
Measles virus - isolation & purification
Measles virus - pathogenicity
Mice
Mice, Inbred Strains
Mutation
Neutralization Tests
Subacute Sclerosing Panencephalitis - mortality
Subacute Sclerosing Panencephalitis - prevention & control
Vero Cells
Viral Plaque Assay
Virus Latency
Virus Replication
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Summary:A small plaque mutant with reduced neurovirulence in newborn mice was obtained from Edmonston strain measles virus after propagation for 5 months in NIH3T3 cells. It retained the antigenicity of the parental virus and tended to induce higher neutralizing antibody titers in the adult BALB/c mice. The intracerebral (but not intraperitoneal) inoculation of the live mutant virus one day before prevented the newborn BALB/c mice from encephalitis caused by the intracerebral challenge with the parental strain at a dose of 10-20 LD50. The intracerebral inoculation with the mutant virus whose replication capacity was inactivated by UV-irradiation was ineffective. The protection was not attributed to interferons nor to viral interference. The mechanism remains unknown.
ISSN:0304-8608
DOI:10.1007/s007050050212