Cyclin‐D1‐gene amplification is a more potent prognostic factor than its protein over‐expression in human head‐and‐neck squamous‐cell carcinoma

• Published in: International journal of cancer 1997-12, Vol.74 (6), p.576-581
• Main Authors: Kyomoto, Ryoichi, Kumazawa, Hirobumi, Toda, Yoshinobu, Sakaida, Noriko, Okamura, Akiharu, Iwanaga, Michitaka, Shintaku, Masayuki, Yamashita, Toshio, Hiai, Hiroshi, Fukumoto, Manabu
• Format: Article
• Language: English
• Subjects: Adult; Aged; Biological and medical sciences; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - metabolism; Carcinoma, Squamous Cell - pathology; Cyclin D1 - biosynthesis; Cyclin D1 - genetics; Female; Gene Amplification; Head and Neck Neoplasms - genetics; Head and Neck Neoplasms - metabolism; Head and Neck Neoplasms - pathology; Humans; Immunohistochemistry; Male; Medical sciences; Middle Aged; Otorhinolaryngology (head neck, general aspects and miscellaneous); Otorhinolaryngology. Stomatology; Paraffin Embedding; Polymerase Chain Reaction; Prognosis; Retrospective Studies; Tumors
• ISSN: 0020-7136; 1097-0215
• DOI: 10.1002/(SICI)1097-0215(19971219)74:6<576::AID-IJC3>3.0.CO;2-R

To evaluate the prognostic significance of cyclin D1 protein/gene expressions in human head‐and‐neck squamous‐cell carcinoma (HNSCC), we examined amplification of the cyclin‐D1 gene (CCND1) by the differential PCR method and over‐expression of cyclin‐D1 protein by immunohistochemistry in 45 paraffin‐embedded sections from HNSCC. Amplification of CCND1 was found in 10 (22%) cases and over‐expression of cyclin D1 was found in 24 (53%) cases. CCND1 amplification was also found in 3 (25%) of 12 cases of dysplastic lesions adjacent to HNSCC. The overall 5‐year survival of patients with CCND1 amplification or with protein over‐production was significantly lower than that of patients without (p < 0.0001 and p < 0.05, respectively). However, with multivariate analysis, only amplification of CCND1 retained an independent prognostic value (p = 0.0018). These suggest that CCND1 amplification occurs at early stages of HNSCC tumorigenesis and is a more useful prognostic factor than over‐expression of cyclin D1 in HNSCC. Int. J. Cancer 74:576–581, 1997.© 1997 Wiley‐Liss, Inc.