Role of E Boxes in the Repression of E-Cadherin Expression

Decreased expression of the intercellular adhesion molecule E-cadherin correlates with tumor aggressiveness and invasion capacity of cell lines. The decrease of E-cadherin expression results primarily from reduced mRNA expression. We show here that the activity of a human E-cadherin promoter constru...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1997-12, Vol.241 (2), p.453-458
Main Authors: Giroldi, Laurence A., Bringuier, Pierre-Paul, de Weijert, Miryam, Jansen, Cornelius, van Bokhoven, Adrie, Schalken, Jack A.
Format: Article
Language:English
Subjects:
Base Sequence
Cadherins - biosynthesis
Cadherins - genetics
Cells, Cultured
DNA Mutational Analysis
Epithelial Cells
Gene Expression Regulation, Neoplastic
Helix-Loop-Helix Motifs
Humans
Molecular Sequence Data
Promoter Regions, Genetic
Sequence Deletion
Tissue Distribution
Transcription Factors - metabolism
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Summary:Decreased expression of the intercellular adhesion molecule E-cadherin correlates with tumor aggressiveness and invasion capacity of cell lines. The decrease of E-cadherin expression results primarily from reduced mRNA expression. We show here that the activity of a human E-cadherin promoter construct is cell specific and correlates with E-cadherin mRNA expression. This spectrum of activity is conserved by a region as short as 81 bp obtained after 5′ deletion. Mutation analysis revealed that two E box elements of this minimal promoter are involved in the silencing of E-cadherin promoter activity occurring in cancer cells. E boxes are mainly known as target sequences for bHLH transcription factors that are involved in the control of tissue differentiation and are antagonised by HLH proteins. However, mutation data and cotransfection experiments using HLH protein expression vectors indicate that bHLH transcription factors are not significantly involved in the E box mediated silencing of this E-cadherin promoter fragment.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1997.7831