Maturation and translation mechanisms involved in the expression of a myb gene of rice

We have isolated two overlapping cDNAs coding for a MYB-related protein expressed in aerobic and anaerobic rice (Oryza sativa) roots and coleoptiles. Analysis of their sequences reveals some peculiar features, suggesting the presence of post-transcriptional regulation events: an upstream ORF, two un...

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Bibliographic Details
Published in:Plant molecular biology 1997-12, Vol.35 (6), p.1003-1008
Main Authors: Magaraggia, F. (Istituto Biosintesi Vegetali CNR, Milano (Italy)), Solinas, G, Valle, G, Giovinazzo, G, Coraggio, I
Format: Article
Language:English
Subjects:
ADN
Anoxia
DNA
EXPRESION GENICA
EXPRESSION DES GENES
FACTEUR DE TRANSCRIPTION
FACTORES DE TRANSCRIPCION
GENE
GENE EXPRESSION
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
GENES
Molecular Sequence Data
Oryza - genetics
Oryza - growth & development
ORYZA SATIVA
Plant Proteins - biosynthesis
Plant Proteins - genetics
Plant Proteins - physiology
Protein Biosynthesis
Rice
Roots
TRANSCRIPTION FACTORS
Transcription Factors - biosynthesis
Transcription Factors - genetics
Transcription Factors - physiology
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Summary:We have isolated two overlapping cDNAs coding for a MYB-related protein expressed in aerobic and anaerobic rice (Oryza sativa) roots and coleoptiles. Analysis of their sequences reveals some peculiar features, suggesting the presence of post-transcriptional regulation events: an upstream ORF, two unspliced introns and a putative leucine zipper in the ORF coded by the unspliced RNA. Transient expression in protoplasts indicates that the upstream ORF inhibits expression of a downstream coding sequence. Finally, we demonstrated that anoxia, in roots, increases the ratio between the spliced and the unspliced mRNA and affects the expression of other myb-related genes.
ISSN:0167-4412
1573-5028
DOI:10.1023/A:1005821518242