In vitro isolation and cultivation of Cowdria ruminantium under serum-free culture conditions

Three stocks of Cowdria ruminantium, the causative agent of heartwater in domestic ruminants, were propagated in bovine endothelial cells in a serum-free culture medium. The Vosloo, Welgevonden and Senegal stocks were propagated for a period of more than 203, 134, and 43 days, respectively. Two of t...

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Bibliographic Details
Published in:Research in veterinary science 1997-09, Vol.63 (2), p.161-164
Main Authors: Zweygarth, E, Vogel, S.W, Josemans, A.I, Horn, E
Format: Article
Language:English
Subjects:
Animals
Cattle
Cattle Diseases - microbiology
Cattle Diseases - pathology
Cell Line
Cowdria ruminantium
Culture Media, Serum-Free
Ehrlichia ruminantium - growth & development
Ehrlichia ruminantium - isolation & purification
Endothelium, Vascular - cytology
Endothelium, Vascular - microbiology
Goat Diseases - microbiology
Goat Diseases - pathology
Goats
Heartwater Disease - microbiology
Heartwater Disease - pathology
Sheep
Sheep Diseases - microbiology
Sheep Diseases - pathology
Time Factors
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Summary:Three stocks of Cowdria ruminantium, the causative agent of heartwater in domestic ruminants, were propagated in bovine endothelial cells in a serum-free culture medium. The Vosloo, Welgevonden and Senegal stocks were propagated for a period of more than 203, 134, and 43 days, respectively. Two of the C ruminantium stocks (Vosloo and Senegal) were also successfully initiated under serum-free culture conditions. The serum-free medium consisted of a modified HL-1 medium. The Senegal stock was successfully propagated in Dulbecco's modified Eagle's medium nutrient mixture Ham F-12 containing 10 per cent fetal bovine serum.
ISSN:0034-5288
1532-2661
DOI:10.1016/S0034-5288(97)90011-4