Isolation and Identification of a Novel Ala-Pro-Gly-Trp-amide-Related Peptide Inhibiting the Motility of the Mature Oviduct in the Cuttlefish, Sepia officinalis

• Published in: Peptides (New York, N.Y. : 1980) N.Y. : 1980), 1997, Vol.18 (10), p.1469-1474
• Main Authors: Henry, Joel, Favrel, Pascal, Boucaud-Camou, Eve
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; APGW-amide; Cephalopods; Chromatography, High Pressure Liquid; Dipeptides - chemistry; Dipeptides - isolation & purification; Dipeptides - metabolism; Dipeptides - pharmacology; Dipeptidyl-Peptidases and Tripeptidyl-Peptidases - metabolism; Female; Mollusca - physiology; Molluscs; Muscle Contraction - drug effects; Neuropeptides; Neuropeptides - chemistry; Neuropeptides - isolation & purification; Neuropeptides - metabolism; Neuropeptides - pharmacology; Optic Lobe, Nonmammalian - chemistry; Oviduct; Oviducts - drug effects; Oviducts - physiology; Protein Processing, Post-Translational; Seasons; Sepia officinalis; Sequence Analysis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• ISSN: 0196-9781; 1873-5169
• DOI: 10.1016/S0196-9781(97)00241-6

Henry J., P. Favrel and E. Boucaud-Camou. Isolation and identification of a novel APGW-amide-related peptide inhibiting the motility of the mature oviduct in the cuttlefish, Sepia officinalis. Peptides 18(10) 1469–1474, 1997.—A novel myotropic neuropeptide was isolated from 110 optic lobes (OL) of mature females of the cuttlefish Sepia officinalis L. by mean of high performance liquid chromatography (HPLC). The peptide inhibits the motility of the oviduct by decreasing the tonus, the frequency and the amplitude of the contractions. The primary structure of the peptide was determined as Gly-Trp-NH 2. This new dipeptide is closely related to the Ala-Pro-Gly-Trp-NH 2 family first identified in gastropod molluscs. On the perfused oviduct, GWa appeared to be 3000 times more potent than APGW-amide. The processing of synthetic APGWa into GWa by diaminopeptidyl activity has been clearly observed in OL extract. Nevertheless, the analysis in MALDI-MS of HPLC OL fractions did not reveal any APGWa related peptides of the known : APGWa, KPGWa, RPGWa and TPGWa. GWa could be processed from a not yet identified APGWa related peptide.