Development, Characterization and Future Prospects of Monoclonal Antibodies Against Spores of Glugea atherinae (Protozoa-Microsporidia-Fish Parasites)

Monoclonal antibodies against spores of Glugea atherinae were obtained after lymphocytic hybridization made from immunized mouse splenocytes. Screening using an indirect enzyme linked immunosorbent assay (ELISA), revealed seven monoclonal antibodies with an intense but variable reaction with the spo...

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Bibliographic Details
Published in:The Journal of eukaryotic microbiology 1997-11, Vol.44 (6), p.643-648
Main Authors: POMPORT-CASTILLON, CECILE, GASC, CHARLES, ROMESTAND, BERNARD
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Antibodies, Protozoan
Antibody Specificity
Binding, Competitive
Biological and medical sciences
Biotinylation
Diagnosis
Female
Fluorescent Antibody Technique, Indirect
Fundamental and applied biological sciences. Psychology
Glugea arherinae
Glugea atherinae
IgM
immunoanalytical tests
Immunoblotting - methods
Mice
Mice, Inbred BALB C
Microsporida - immunology
Microsporidia
monoclonal antibodies
Protozoa
Spores
Systematics. Geographical distribution. Morphology. Cytology
taxonomy
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Summary:Monoclonal antibodies against spores of Glugea atherinae were obtained after lymphocytic hybridization made from immunized mouse splenocytes. Screening using an indirect enzyme linked immunosorbent assay (ELISA), revealed seven monoclonal antibodies with an intense but variable reaction with the spores of fish microsporidia, and a moderate reaction with those of an insect microsporidium (Nosema sp.). The reaction was weaker with spores of Encephalitozoon intestinalis found in HIV' patients. FITC and Dot Blot confirmed the majority of these results. After biotinylation of the seven antibodies, inhibition tests allowed the localization of two different recognition domains on the spores of Glugea atherinae. The multiple antigenic determinants and their probable polysaccharide nature seem to be in accord with the class IgM of the antibodies produced. This work confirms the potential of these antibodies for microsporidian taxonomy and diagnosis, especially the use of Mabs 12F9 and 12H5 for detection of spores in stools of HIV+ patients.
ISSN:1066-5234
1550-7408
DOI:10.1111/j.1550-7408.1997.tb05972.x