Damaged chromatin does not prevent the exit from metaphase I in fused mouse oocytes

The presence of checkpoint mechanisms which are able to recognize damaged chromatin and thereafter to prevent exit from metaphase I has been investigated in giant mouse oocytes produced by fusion of a normal metaphase I oocyte with an equivalent oocyte with damaged chromatin. The presence of damaged...

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Bibliographic Details
Published in:Human reproduction (Oxford) 1997-11, Vol.12 (11), p.2473-2476
Main Authors: Fulka, J., Kalab, P., First, N.L., Moor, R.M.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell cycle, cell proliferation
Cell Fusion
Cell physiology
checkpoint
Chromatin - pathology
chromosomes
Female
Fundamental and applied biological sciences. Psychology
Metaphase
Mice
Molecular and cellular biology
oocytes
Oocytes - pathology
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Summary:The presence of checkpoint mechanisms which are able to recognize damaged chromatin and thereafter to prevent exit from metaphase I has been investigated in giant mouse oocytes produced by fusion of a normal metaphase I oocyte with an equivalent oocyte with damaged chromatin. The presence of damaged chromatin did not prevent the onset of anaphase I in both sets of chromatin in the fused cells. Interestingly, fused or unfused cells containing only damaged chromatin failed to enter anaphase and persisted instead in a metaphase-like state. These results demonstrate the fragility of checkpoint controls in mammalian female germ cells.
ISSN:0268-1161
1460-2350
DOI:10.1093/humrep/12.11.2473