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Defect in Th1-Like Cells of Nonresponders to Hepatitis B Vaccine
Peripheral blood lymphocytes from nonresponders to hepatitis B vaccine (HBsAg) failed to undergo a proliferative response to recombinant HBsAg in vitro, whereas cells from responders proliferated vigorously. The lack of proliferative response was not due to defective antigen presentation in that MHC...
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| Published in: | Human immunology 1997-11, Vol.58 (1), p.42-51 |
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| Main Authors: | , , , , , , , , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c443t-469faf52a64f77506e1ac7c5138e6c51a6c465d7299bdc7fc09441605d613b5e3 |
| container_end_page | 51 |
| container_issue | 1 |
| container_start_page | 42 |
| container_title | Human immunology |
| container_volume | 58 |
| creator | Chedid, Marie G Deulofeut, Harold Yunis, David E Lara-Marquez, Maria Luz Salazar, Marcela Deulofeut, Richard Awdeh, Zuheir Alper, Chester A Yunis, Edmond J |
| description | Peripheral blood lymphocytes from nonresponders to hepatitis B vaccine (HBsAg) failed to undergo a proliferative response to recombinant HBsAg
in vitro, whereas cells from responders proliferated vigorously. The lack of proliferative response was not due to defective antigen presentation in that MHC-identical responder and nonresponder antigen presenting cells were equally effective in stimulating responder T cells. Nonresponder T cells did not proliferate in response to antigen-pulsed MHC identical responder antigen presenting cells. The present study demonstrated that: 1) there were no detectable (1 in |
| doi_str_mv | 10.1016/S0198-8859(97)00209-7 |
| format | article |
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in vitro, whereas cells from responders proliferated vigorously. The lack of proliferative response was not due to defective antigen presentation in that MHC-identical responder and nonresponder antigen presenting cells were equally effective in stimulating responder T cells. Nonresponder T cells did not proliferate in response to antigen-pulsed MHC identical responder antigen presenting cells. The present study demonstrated that: 1) there were no detectable (1 in <20 × 10
4) HBsAg-precursor T cells in any of the nonresponders, while in responders the frequency of HBsAg-precursor T cells ranged from 1 in 3.2 × 10
3 to 1 in 40 × 10
3; 2) nonresponder cell cultures did not secrete IL-2 in response to HBsAg stimulation; 3) exogenous recombinant IL-2 did not restore the proliferative response of the T cells in HBsAg-pulsed cultures of nonresponders.
These results suggest that the cellular basis for the lack of response to HBsAg is a defect in HBsAg-specific Th1-like cells; either there is an absence of the Th1 cells or cells with TCR specificity for HBsAg are present but are unresponsive to the HBsAg peptide-MHC complex (
i.e., anergy or tolerance).</description><identifier>ISSN: 0198-8859</identifier><identifier>EISSN: 1879-1166</identifier><identifier>DOI: 10.1016/S0198-8859(97)00209-7</identifier><identifier>PMID: 9438208</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult ; Aged ; AIDS/HIV ; Cells, Cultured ; Hepatitis B Surface Antigens - immunology ; Hepatitis B Vaccines - immunology ; Humans ; Interleukin-2 - immunology ; Interleukin-2 - pharmacology ; Middle Aged ; Phytohemagglutinins - immunology ; Tetanus Toxoid - immunology ; Th1 Cells - immunology ; Vaccines, Synthetic - immunology</subject><ispartof>Human immunology, 1997-11, Vol.58 (1), p.42-51</ispartof><rights>1997 American Society for Histocompatibility and Immunogenetics</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-469faf52a64f77506e1ac7c5138e6c51a6c465d7299bdc7fc09441605d613b5e3</citedby><cites>FETCH-LOGICAL-c443t-469faf52a64f77506e1ac7c5138e6c51a6c465d7299bdc7fc09441605d613b5e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9438208$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chedid, Marie G</creatorcontrib><creatorcontrib>Deulofeut, Harold</creatorcontrib><creatorcontrib>Yunis, David E</creatorcontrib><creatorcontrib>Lara-Marquez, Maria Luz</creatorcontrib><creatorcontrib>Salazar, Marcela</creatorcontrib><creatorcontrib>Deulofeut, Richard</creatorcontrib><creatorcontrib>Awdeh, Zuheir</creatorcontrib><creatorcontrib>Alper, Chester A</creatorcontrib><creatorcontrib>Yunis, Edmond J</creatorcontrib><title>Defect in Th1-Like Cells of Nonresponders to Hepatitis B Vaccine</title><title>Human immunology</title><addtitle>Hum Immunol</addtitle><description>Peripheral blood lymphocytes from nonresponders to hepatitis B vaccine (HBsAg) failed to undergo a proliferative response to recombinant HBsAg
in vitro, whereas cells from responders proliferated vigorously. The lack of proliferative response was not due to defective antigen presentation in that MHC-identical responder and nonresponder antigen presenting cells were equally effective in stimulating responder T cells. Nonresponder T cells did not proliferate in response to antigen-pulsed MHC identical responder antigen presenting cells. The present study demonstrated that: 1) there were no detectable (1 in <20 × 10
4) HBsAg-precursor T cells in any of the nonresponders, while in responders the frequency of HBsAg-precursor T cells ranged from 1 in 3.2 × 10
3 to 1 in 40 × 10
3; 2) nonresponder cell cultures did not secrete IL-2 in response to HBsAg stimulation; 3) exogenous recombinant IL-2 did not restore the proliferative response of the T cells in HBsAg-pulsed cultures of nonresponders.
These results suggest that the cellular basis for the lack of response to HBsAg is a defect in HBsAg-specific Th1-like cells; either there is an absence of the Th1 cells or cells with TCR specificity for HBsAg are present but are unresponsive to the HBsAg peptide-MHC complex (
i.e., anergy or tolerance).</description><subject>Adult</subject><subject>Aged</subject><subject>AIDS/HIV</subject><subject>Cells, Cultured</subject><subject>Hepatitis B Surface Antigens - immunology</subject><subject>Hepatitis B Vaccines - immunology</subject><subject>Humans</subject><subject>Interleukin-2 - immunology</subject><subject>Interleukin-2 - pharmacology</subject><subject>Middle Aged</subject><subject>Phytohemagglutinins - immunology</subject><subject>Tetanus Toxoid - immunology</subject><subject>Th1 Cells - immunology</subject><subject>Vaccines, Synthetic - immunology</subject><issn>0198-8859</issn><issn>1879-1166</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNqFkElPwzAQhS0EKqXwEyr5hOAQsBMv8YmlLEWq4EDharnORBjaONgpEv8ed1GvnObw3ps38yE0pOSCEiouXwlVZVaWXJ0peU5ITlQm91CfllJllAqxj_o7yyE6ivGTECKJZD3UU6woc1L20fUd1GA77Bo8_aDZxH0BHsF8HrGv8bNvAsTWNxWEiDuPx9CaznUu4lv8bqx1DRyjg9rMI5xs5wC9PdxPR-Ns8vL4NLqZZJaxosuYULWpeW4Eq6XkRAA1VlpOixJEGkZYJnglc6VmlZW1JYoxKgivBC1mHIoBOt3sbYP_XkLs9MJFmy41Dfhl1FLxXPKc_2ukIr3OFE1GvjHa4GMMUOs2uIUJv5oSvUKs14j1ip9WUq8Ra5lyw23BcraAapfaMk361UaHhOPHQdDROmgsVC4k1Lry7p-GP-SkiPs</recordid><startdate>19971101</startdate><enddate>19971101</enddate><creator>Chedid, Marie G</creator><creator>Deulofeut, Harold</creator><creator>Yunis, David E</creator><creator>Lara-Marquez, Maria Luz</creator><creator>Salazar, Marcela</creator><creator>Deulofeut, Richard</creator><creator>Awdeh, Zuheir</creator><creator>Alper, Chester A</creator><creator>Yunis, Edmond J</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19971101</creationdate><title>Defect in Th1-Like Cells of Nonresponders to Hepatitis B Vaccine</title><author>Chedid, Marie G ; Deulofeut, Harold ; Yunis, David E ; Lara-Marquez, Maria Luz ; Salazar, Marcela ; Deulofeut, Richard ; Awdeh, Zuheir ; Alper, Chester A ; Yunis, Edmond J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-469faf52a64f77506e1ac7c5138e6c51a6c465d7299bdc7fc09441605d613b5e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Adult</topic><topic>Aged</topic><topic>AIDS/HIV</topic><topic>Cells, Cultured</topic><topic>Hepatitis B Surface Antigens - immunology</topic><topic>Hepatitis B Vaccines - immunology</topic><topic>Humans</topic><topic>Interleukin-2 - immunology</topic><topic>Interleukin-2 - pharmacology</topic><topic>Middle Aged</topic><topic>Phytohemagglutinins - immunology</topic><topic>Tetanus Toxoid - immunology</topic><topic>Th1 Cells - immunology</topic><topic>Vaccines, Synthetic - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chedid, Marie G</creatorcontrib><creatorcontrib>Deulofeut, Harold</creatorcontrib><creatorcontrib>Yunis, David E</creatorcontrib><creatorcontrib>Lara-Marquez, Maria Luz</creatorcontrib><creatorcontrib>Salazar, Marcela</creatorcontrib><creatorcontrib>Deulofeut, Richard</creatorcontrib><creatorcontrib>Awdeh, Zuheir</creatorcontrib><creatorcontrib>Alper, Chester A</creatorcontrib><creatorcontrib>Yunis, Edmond J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Human immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chedid, Marie G</au><au>Deulofeut, Harold</au><au>Yunis, David E</au><au>Lara-Marquez, Maria Luz</au><au>Salazar, Marcela</au><au>Deulofeut, Richard</au><au>Awdeh, Zuheir</au><au>Alper, Chester A</au><au>Yunis, Edmond J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Defect in Th1-Like Cells of Nonresponders to Hepatitis B Vaccine</atitle><jtitle>Human immunology</jtitle><addtitle>Hum Immunol</addtitle><date>1997-11-01</date><risdate>1997</risdate><volume>58</volume><issue>1</issue><spage>42</spage><epage>51</epage><pages>42-51</pages><issn>0198-8859</issn><eissn>1879-1166</eissn><abstract>Peripheral blood lymphocytes from nonresponders to hepatitis B vaccine (HBsAg) failed to undergo a proliferative response to recombinant HBsAg
in vitro, whereas cells from responders proliferated vigorously. The lack of proliferative response was not due to defective antigen presentation in that MHC-identical responder and nonresponder antigen presenting cells were equally effective in stimulating responder T cells. Nonresponder T cells did not proliferate in response to antigen-pulsed MHC identical responder antigen presenting cells. The present study demonstrated that: 1) there were no detectable (1 in <20 × 10
4) HBsAg-precursor T cells in any of the nonresponders, while in responders the frequency of HBsAg-precursor T cells ranged from 1 in 3.2 × 10
3 to 1 in 40 × 10
3; 2) nonresponder cell cultures did not secrete IL-2 in response to HBsAg stimulation; 3) exogenous recombinant IL-2 did not restore the proliferative response of the T cells in HBsAg-pulsed cultures of nonresponders.
These results suggest that the cellular basis for the lack of response to HBsAg is a defect in HBsAg-specific Th1-like cells; either there is an absence of the Th1 cells or cells with TCR specificity for HBsAg are present but are unresponsive to the HBsAg peptide-MHC complex (
i.e., anergy or tolerance).</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>9438208</pmid><doi>10.1016/S0198-8859(97)00209-7</doi><tpages>10</tpages></addata></record> |
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| ispartof | Human immunology, 1997-11, Vol.58 (1), p.42-51 |
| issn | 0198-8859 1879-1166 |
| language | eng |
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| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | Adult Aged AIDS/HIV Cells, Cultured Hepatitis B Surface Antigens - immunology Hepatitis B Vaccines - immunology Humans Interleukin-2 - immunology Interleukin-2 - pharmacology Middle Aged Phytohemagglutinins - immunology Tetanus Toxoid - immunology Th1 Cells - immunology Vaccines, Synthetic - immunology |
| title | Defect in Th1-Like Cells of Nonresponders to Hepatitis B Vaccine |
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