Epidermal growth factor increases the labeling of phosphatidylinositol 3,4-bisphosphate in MA-10 Leydig tumor cells

Previous studies from this laboratory have shown that mouse epidermal growth factor (mEGF) modulates the hormonal responsiveness of MA-10 Leydig tumor cells without affecting cell multiplication. In an attempt to characterize the intracellular signaling systems activated by mEGF in this cell type, w...

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Bibliographic Details
Published in:The Journal of biological chemistry 1990-01, Vol.265 (3), p.1718-1723
Main Authors: Pignataro, O P, Ascoli, M
Format: Article
Language:English
Subjects:
Animals
Arginine Vasopressin - pharmacology
Cell Line
Chorionic Gonadotropin - pharmacology
Chromatography, High Pressure Liquid
Epidermal Growth Factor - pharmacology
In Vitro Techniques
Leydig Cell Tumor - metabolism
Mice
Phosphatidylinositols - metabolism
Rats
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Summary:Previous studies from this laboratory have shown that mouse epidermal growth factor (mEGF) modulates the hormonal responsiveness of MA-10 Leydig tumor cells without affecting cell multiplication. In an attempt to characterize the intracellular signaling systems activated by mEGF in this cell type, we examined its effects on the labeling of phosphatidylinositols in cells that had been preincubated with different radioactive precursors. Here we report that exposure of MA-10 cells to mEGF, but not other ligands that affect their differentiated function, results in an increase in the labeling of an unusual phosphatidylinositol that does not appear to be present in unstimulated cells. This phosphatidylinositol has been identified as phosphatidylinositol 3,4-bisphosphate.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(19)40075-6