Molecular and phenotypic characterization of Aspergillus japonicus and Aspergillus aculeatus strains with special regard to their mitochondrial DNA polymorphisms

Forty Aspergillus japonicus and A. aculeatus strains, most of them wild-type isolates, were examined using various molecular and phenotypic techniques. The rDNAs proved to be invariable (even strains of the species A. aculeatus exhibited the same restriction profile), while the strains could be clas...

Full description

Saved in:
Bibliographic Details
Published in:Antonie van Leeuwenhoek 1997-11, Vol.72 (4), p.337-347
Main Authors: HAMARI, Z, KEVEI, F, KOVACS, E, VARGA, J, KOZAKIEWICZ, Z, CROFT, J. H
Format: Article
Language:English
Subjects:
Aspergillus - enzymology
Aspergillus - genetics
Aspergillus - metabolism
Biological and medical sciences
Carbon - metabolism
Carbon sources
Cell Nucleus - genetics
DNA, Fungal - analysis
DNA, Mitochondrial - analysis
DNA, Ribosomal - analysis
Fundamental and applied biological sciences. Psychology
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Isoenzymes - analysis
Microbiology
Mitochondrial DNA
Mycology
Phenotype
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
Random Amplified Polymorphic DNA Technique
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Forty Aspergillus japonicus and A. aculeatus strains, most of them wild-type isolates, were examined using various molecular and phenotypic techniques. The rDNAs proved to be invariable (even strains of the species A. aculeatus exhibited the same restriction profile), while the strains could be classified into seven different mtDNA RFLP groups. Hybridisation data suggest that six of these mtDNA types have certain common restriction sites, while mtDNA type 7, which was exhibited by some A. aculeatus strains, probably has quite different mtDNA organisation and their size was smallest among the strains studied. The RAPD technique and isoenzyme analysis revealed some variabilities within these RFLP groups and strain specific features could also be recognised. Carbon source assimilation spectra were found to be very distinctive for strains of A. japonicus, A. aculeatus and A. niger, providing a useful tool for pre-characterising new wild-type isolates of black Aspergilli. Only a limited correlation was observed between the dendrograms based on genotypic and phenotypic characters.
ISSN:0003-6072
1572-9699
DOI:10.1023/A:1000578913759