Analysis of haemorrhagic septicaemia-causing isolates of Pasteurella multocida by ribotyping and field alternation gel electrophoresis (FAGE)

Ribotyping and field alternation gel electrophoresis (FAGE) were used to examine 19 Pasteurella multocida isolates, and to assess the ability of these techniques to differentiate P. multocida strains that cause haemorrhagic septicaemia (HS). Reproducible patterns were obtained from both methods, wit...

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Bibliographic Details
Published in:Veterinary microbiology 1997-10, Vol.57 (4), p.383-395
Main Authors: Townsend, K.M., Dawkins, H.J.S., Papadimitriou, J.M.
Format: Article
Language:English
Subjects:
Animals
Bacteriology
Biological and medical sciences
Bison
Buffaloes
Cattle
Cattle Diseases
Deer
Deoxyribonucleases, Type II Site-Specific
DNA, Bacterial - analysis
Electrophoresis
Field alternation gel electrophoresis
Fundamental and applied biological sciences. Psychology
Genetic Techniques
Haemorrhagic septicaemia
Hemorrhagic Septicemia - microbiology
Hemorrhagic Septicemia - veterinary
Humans
Microbiology
Pasteurella Infections - microbiology
Pasteurella Infections - veterinary
Pasteurella multocida
Pasteurella multocida - classification
Pasteurella multocida - isolation & purification
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Polymorphism, Restriction Fragment Length
Reproducibility of Results
Restriction fragment length polymorphism
Ribotyping
Serotyping
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Summary:Ribotyping and field alternation gel electrophoresis (FAGE) were used to examine 19 Pasteurella multocida isolates, and to assess the ability of these techniques to differentiate P. multocida strains that cause haemorrhagic septicaemia (HS). Reproducible patterns were obtained from both methods, with FAGE demonstrating greater discriminatory power than ribotyping. FAGE analysis was particularly useful in distinguishing North American cultures originating from the 1922 Yellowstone National Park Buffalo ‘B’ strain, demonstrating the ability to detect genetic alterations induced by repeated subculture. A remarkable homogeneity was observed among Asian HS strains following ribotyping and FAGE analysis, with a clear distinction observed between virulent and avirulent HS isolates. This study has illustrated the value of genomic fingerprinting methods in distinguishing strains of similar serotype, and the capability of these methods to produce detailed characterisation of P. multocida isolates.
ISSN:0378-1135
1873-2542
DOI:10.1016/S0378-1135(97)00121-1